Tavakoli Sahar, Zhu Shiwen, Matsudaira Paul
Center for BioImaging Sciences, Department of Biological Sciences, Mechanobiology Institute, National University of Singapore, Singapore 119077, Singapore.
iScience. 2022 Apr 22;25(5):104280. doi: 10.1016/j.isci.2022.104280. eCollection 2022 May 20.
In the mammalian intestine, stem cells (ISCs) replicate in basal crypts, translocate along the villus, and undergo cell death. This pattern of renewal occurs in the zebrafish intestine in which villi are elongated into villar ridges (VR) separated by intervillus pockets (IVP) but lack the infolded crypts. To understand how epithelial dynamics is maintained without crypts, we investigated the origin of epithelial lineage patterns derived from ISCs in the IVP of chimeric and zebrabow recombinant intestines. We found that the VR epithelium and IVP express the same recombinant colors when expression is under the control of ISC marker promoter . The expression originates from cell clusters that line the IVP and contain epithelial cells including Prmt1-labeled cells. Our data suggest that Prmt1 is a zebrafish ISC marker and the ISCs reside within basal cell clusters that are functionally analogous to crypts.
在哺乳动物肠道中，干细胞（ISC）在隐窝底部复制，沿绒毛迁移，并经历细胞死亡。这种更新模式也出现在斑马鱼肠道中，其绒毛延伸为被绒毛间袋（IVP）隔开的绒毛嵴（VR），但没有内陷的隐窝。为了了解在没有隐窝的情况下上皮动力学是如何维持的，我们研究了嵌合和斑马鱼重组肠道IVP中源自ISC的上皮谱系模式的起源。我们发现，当表达受ISC标记启动子控制时，VR上皮和IVP表达相同的重组颜色。该表达源自排列在IVP内并包含包括Prmt1标记细胞在内的上皮细胞的细胞簇。我们的数据表明，Prmt1是斑马鱼ISC标记，ISC存在于功能上类似于隐窝的基底细胞簇中。
