Buck R H, Krummen K
J Chromatogr. 1987 Jan 30;387:255-65. doi: 10.1016/s0021-9673(01)94529-7.
o-Phthaldialdehyde in combination with a chiral mercaptan is a powerful chiral reagent for the pre-column derivatization of many enantiomeric compounds bearing primary amino groups. The diastereoisomers formed can efficiently be resolved on conventional reversed-phase columns. Simultaneous determination of the enantiomers of various amino acids, amino alcohols and biogenic amines was achieved by gradient elution and fluorescence detection. The resolution was optimized by varying the chiral mercaptan in the reagent, Boc-L-cysteine, N-acetyl-L-cysteine and N-acetyl-D-penicillamine being used for this purpose. The resolutions were calculated. Most of the enantiomers showed good resolution with each of the three chiral mercaptans, whereas some enantiomers were only separable by one or two of them. The method was applied to the analysis of peptide hydrolysates. The composition of peptides bearing L- and D-amino acids and an amino alcohol was determined.
邻苯二甲醛与手性硫醇联用是一种强大的手性试剂，用于许多带有伯氨基的对映体化合物的柱前衍生化。形成的非对映异构体可在常规反相柱上有效分离。通过梯度洗脱和荧光检测实现了对各种氨基酸、氨基醇和生物胺对映体的同时测定。通过改变试剂中的手性硫醇来优化分离度，为此使用了Boc-L-半胱氨酸、N-乙酰-L-半胱氨酸和N-乙酰-D-青霉胺。计算了分离度。大多数对映体与三种手性硫醇中的每一种都显示出良好的分离度，而一些对映体仅可被其中一种或两种分离。该方法应用于肽水解产物的分析。测定了含有L-和D-氨基酸以及一种氨基醇的肽的组成。
