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酿酒酵母自然菌株中的“杀手”质粒

["Killer" plasmid in natural strains of Saccharomyces].

作者信息

Nesterova G F, Milovatskiĭ V S, Sizova N I, Semykina L V

出版信息

Mol Biol (Mosk). 1978 Jul-Aug;12(4):781-6.

PMID:355864
Abstract

Double stranded RNA's of thirteen wine strains of Saccharomyces belonging to different species and exhibiting killing activity were studied. Two strains of Oxford genetic stocks were used for control. All these strains have four different spectra of killer activity and contain two double stranded RNAs, L and M. All strains regardless of the type of killer activity have the same electrophoretic mobility of L. The mobility of M varies in different strains but is not connected with the spectrum of killing activity. All strains can be cured by cycloheximide. Cured variants have no M. Certain cured strains contain small portions of double stranded RNAs with electrophoretical mobility faster than of M.

摘要

对属于不同物种且具有杀伤活性的13株酿酒酵母的双链RNA进行了研究。使用了两株牛津遗传菌株作为对照。所有这些菌株具有四种不同的杀伤活性谱,并且含有两种双链RNA,L和M。所有菌株无论杀伤活性类型如何,L的电泳迁移率都相同。M的迁移率在不同菌株中有所不同,但与杀伤活性谱无关。所有菌株都可以用环己酰亚胺治愈。治愈的变体没有M。某些治愈的菌株含有电泳迁移率比M快的双链RNA小片段。

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