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[酵母酿酒酵母中的染色体多态性]

[Chromosome polymorphism in the yeast Saccharomyces].

作者信息

Davydenko S G, Milovatskiĭ V S, Nesterova G F, Skliarova L L, Mezhevaia E V, Iarovoĭ B F

出版信息

Genetika. 1990 Dec;26(12):2135-46.

PMID:2086343
Abstract

The variability of chromosomal band patterns was determined by pulse electrophoresis. The natural strains differed by the quantity and electrophoretic mobility of chromosomal DNA bands. The strains of independent genetic stocks originated from the XII race of Saccharomyces cerevisiae showed less significant difference in band patterns than the strains of different species of the Saccharomyces genus. The progeny of among strains with different karyotypes hybrid showed non-regular segregation of parental bands, the occurrence of new bands and the bands with altered mobility. Reverse crosses of hybrid progeny with strains of Peterhoff genetic stocks of S. cerevisiae led to decrease in chromosomal polymorphism. Homozygotization for ski5 allele and selection for increasing the copy number of killer plasmids was accompanied with repeated splash of polymorphism in 1-2 generations of intratetrad and intrafamily crossed hybrid progeny. Subsequent stabilization of electrophoretic karyotype took place, excluding the mendelian dimorphism of chromosome III, with was a stable trait of the last 6 generations of that progeny.

摘要

通过脉冲电泳确定染色体带型的变异性。自然菌株在染色体DNA带的数量和电泳迁移率方面存在差异。源自酿酒酵母XII族的独立遗传株系的菌株在带型上的差异比酿酒酵母属不同物种的菌株小。不同核型杂交菌株的后代显示出亲本带的不规则分离、新带的出现以及迁移率改变的带。杂种后代与酿酒酵母彼得霍夫遗传株系的菌株进行反交导致染色体多态性降低。ski5等位基因的纯合化以及增加杀伤质粒拷贝数的选择伴随着1-2代四分体内部和家族内部杂交杂种后代多态性的反复波动。随后电泳核型发生稳定,排除了III号染色体的孟德尔二态性,这是该后代最后6代的稳定特征。

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