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[分析微生物杂交DNA分子的热稳定性作为提高分子杂交技术分辨率的一种手段]

[Analysis of the thermostability of the hybrid DNA molecules of microorganisms as a means of increasing the resolution of the molecular hybridization technic].

作者信息

Mednikov B M, Turova T P

出版信息

Mol Biol (Mosk). 1978 Jul-Aug;12(4):853-6.

PMID:355865
Abstract

A comparative study of termostability of microorganisms DNA was performed in order to increase the resolution of the method of molecular hybridization. Molecular hybridization was carried out and the curve of hybrid DNA duplexes distribution, acccording to termostability of two groups of microorganisms, related to strains Echerichia coli B1 and Vibrio cholerae eltor 18647, were obtained. It was determined that the form of the curves is specie specific for the microorganisms investigated but there exists a similarity between the closely related strains which can not be distinguished by the percent of homology.

摘要

为提高分子杂交方法的分辨率,对微生物DNA的热稳定性进行了比较研究。进行了分子杂交,并根据与大肠杆菌B1株和霍乱弧菌埃尔托18647株相关的两组微生物的热稳定性,获得了杂交DNA双链分布曲线。结果表明,曲线形式对所研究的微生物具有种特异性,但密切相关的菌株之间存在相似性,无法通过同源性百分比来区分。

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