Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Minhang 200240, Shanghai, China.
Department of Plant and Environmental Sciences (PLEN), University of Copenhagen, 1870 Frederiksberg, Denmark.
Development. 2022 Jun 15;149(12). doi: 10.1242/dev.200415. Epub 2022 Jun 21.
Rice (Oryza sativa) is one of our main food crops, feeding ∼3.5 billion people worldwide. An increasing number of studies note the importance of the cytoskeleton, including actin filaments and microtubules, in rice development and environmental responses. Yet, reliable in vivo cytoskeleton markers are lacking in rice, which limits our knowledge of cytoskeletal functions in living cells. Therefore, we generated bright fluorescent marker lines of the actin and microtubule cytoskeletons in rice, suitable for live-cell imaging in a wide variety of rice tissues. Using these lines, we show that actin bundles and microtubules engage and co-function during pollen grain development, how the cytoskeletal components are coordinated during root cell development, and that the actin cytoskeleton is robust and facilitates microtubule responses during salt stress. Hence, we conclude that our cytoskeletal marker lines, highlighted by our findings of cytoskeletal associations and dynamics, will substantially further future investigations in rice biology.
水稻（Oryza sativa）是我们的主要粮食作物之一，为全球约 35 亿人提供食物。越来越多的研究指出细胞骨架（包括肌动蛋白丝和微管）在水稻发育和环境响应中的重要性。然而，在水稻中缺乏可靠的细胞骨架活体标记物，这限制了我们对活细胞中细胞骨架功能的了解。因此，我们在水稻中生成了肌动蛋白和微管细胞骨架的明亮荧光标记线，适合在各种水稻组织中进行活细胞成像。利用这些标记线，我们展示了在花粉粒发育过程中肌动蛋白束和微管的结合和共同作用，细胞骨架成分在根细胞发育过程中的协调方式，以及肌动蛋白细胞骨架的稳健性及其在盐胁迫过程中对微管响应的促进作用。因此，我们得出结论，我们的细胞骨架标记线，突出了细胞骨架的关联和动态，将极大地促进未来对水稻生物学的研究。
