Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Department of Pharmaceutical Biomaterials, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
J Biomed Mater Res B Appl Biomater. 2022 Nov;110(11):2411-2421. doi: 10.1002/jbm.b.35087. Epub 2022 May 19.
In this study, polyvinyl alcohol hydrogel chains were crosslinked by polyurethane in order to synthesize a suitable substrate for cartilage lesions. The substrate was fully characterized, and in vitro and in vivo investigations were conducted based on a sheep model. In vitro tests were performed based on the chondrocyte cells with the Alcian Blue and safranin O staining in order to prove the presence of proteoglycan on the surface of the synthesized substrate, which has been secreted by cultures of chondrocytes. Furthermore, the expression of collagen type I, collagen type II, aggrecan, and Sox9 was presented in the chondrocyte cultures on the synthesized substrate through RT-PCR. In addition, the H&E analysis and other related tests demonstrated the formation of neocartilage tissue in a sheep model. The results were found to be promising for cartilage tissue engineering and verified that the isolated chondrocyte cultures on the synthesized substrate retain their original composition.
在这项研究中，通过聚氨酯使聚乙烯醇水凝胶链交联，以合成一种适合软骨损伤的基质。对该基质进行了全面的表征，并基于绵羊模型进行了体外和体内研究。体外试验是基于软骨细胞进行的，通过阿尔辛蓝和番红 O 染色来证明合成基质表面存在由软骨细胞培养物分泌的蛋白聚糖。此外，通过 RT-PCR 展示了在合成基质上的软骨细胞培养物中存在的 I 型胶原、II 型胶原、聚集蛋白聚糖和 Sox9 的表达。此外，H&E 分析和其他相关测试表明在绵羊模型中形成了新的软骨组织。结果对于软骨组织工程具有很大的前景，并验证了在合成基质上分离的软骨细胞培养物保持其原始组成。
