National Cell Bank of Iran, Pasteur Institute of Iran, 13164, Tehran, Iran,
J Mater Sci Mater Med. 2013 Oct;24(10):2449-60. doi: 10.1007/s10856-013-4995-1. Epub 2013 Jun 27.
Polyvinyl alcohol (PVA) hydrogel chains were crosslinked by urethane pre-polymer (PPU) in order to fabricate a new substitute for cartilage lesions. The microscopy images showed that the cultured chondrocytes had spherical morphology on PVA-PPU sample after 4 weeks of isolation in vitro. The alcian blue and safranin O staining proved the presence of proteoglycan on the surface of PVA-PPU sample secreted by cultured chondrocytes. This was confirmed by the detection of sulfate ions in the wavelength dispersive X-ray (WDX) analysis. In addition, the expression of collagen type II and aggrecan were observed in chondrocytes cultured on PVA-PPU by RT-PCR. Moreover, the implantation of the PVA-PPU sample with autologous cultured chondrocytes revealed the formation of neocartilage tissue in a rabbit model during 12 weeks follow up. In conclusion, the results verified that isolated chondrocytes cultured on PVA-PPU retain their original phenotype and this composition can be considered as promising substrate for cartilage tissue engineering.
聚乙烯醇（PVA）水凝胶链通过氨酯预聚物（PPU）交联，以制造软骨病变的新替代品。显微镜图像显示，在体外培养 4 周后，培养的软骨细胞在 PVA-PPU 样品上具有球形形态。阿利新蓝和番红 O 染色证明了培养的软骨细胞在 PVA-PPU 样品表面分泌的蛋白聚糖的存在。这通过波长分散 X 射线（WDX）分析中硫酸根离子的检测得到证实。此外，通过 RT-PCR 观察到培养在 PVA-PPU 上的软骨细胞中 II 型胶原和聚集蛋白聚糖的表达。此外，将自体培养的软骨细胞植入 PVA-PPU 样品中，在 12 周的随访中，在兔模型中显示出新生软骨组织的形成。总之，结果证实，培养在 PVA-PPU 上的分离软骨细胞保留其原始表型，并且这种成分可以被认为是软骨组织工程有前途的基质。
