Institute of Solid State Physics, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui 230031, China.
Department of Chemistry, University of Science and Technology of China, Hefei 230026, China.
Anal Chem. 2022 May 31;94(21):7559-7566. doi: 10.1021/acs.analchem.2c00380. Epub 2022 May 19.
Mesna is an important regional antidote for protecting the urinary system of chemotherapy patients, which requires monitoring its level in real time to ensure the curative effect. The fluorescence method is a powerful tool in real-time detection with the advantages of fast response and visualization. However, the background interference limits its application in biological sensing. Here, we developed a portable sensing platform using an upconversion-based nanosensor for visual quantitative monitoring of mesna in real-time/on-site conditions. The nanosensor was constructed by upconversion nanoparticles (UCNPs) and ethyl violet (EV), in which the UCNPs emitted red and green light, while EV quenched the green light due to the inner filter effect (IFE). The reaction of mesna with EV caused its fading and broke the IFE process, leading to the recovery of green light. By the fluorescence and colorimetric chromaticity variations, the nanosensor achieved a dual-readout detection for mesna with low limits of detection (LODs) of 26 and 48 nM, respectively. Furthermore, a highly compatible sensing platform was fabricated for facile determination of mesna with an LOD of 56 nM, realizing visual quantitative monitoring of the mesna level to ensure the curative effect and providing a new strategy for point-of-care testing of drugs in clinical settings.
美司钠是一种保护化疗患者泌尿系统的重要区域性解毒剂，需要实时监测其水平以确保疗效。荧光法是实时检测的有力工具，具有快速响应和可视化的优点。然而，背景干扰限制了其在生物传感中的应用。在这里，我们开发了一种基于上转换的纳米传感器的便携式传感平台，用于实时/现场条件下可视化定量监测美司钠。该纳米传感器由上转换纳米粒子 (UCNPs) 和乙基紫 (EV) 构建，其中 UCNPs 发射红光和绿光，而 EV 由于内滤效应 (IFE) 而使绿光猝灭。美司钠与 EV 的反应导致其褪色并破坏 IFE 过程，从而恢复绿光。通过荧光和比色色度变化，纳米传感器实现了对美司钠的双读取检测，检测限 (LOD) 分别低至 26 和 48 nM。此外，还构建了一种高度兼容的传感平台，用于简便地测定美司钠，LOD 为 56 nM，实现了美司钠水平的可视化定量监测，以确保疗效，并为临床环境中药物的即时检测提供了新策略。
