Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, PR China.
College of Medical Engineering, Jining Medical University, Jining, 272067, PR China.
Talanta. 2022 Apr 1;240:123165. doi: 10.1016/j.talanta.2021.123165. Epub 2021 Dec 22.
As one of primary biomarkers of ovarian cancer in early stages, β-galactosidase (β-Gal) is significant in the discovery and diagnosis of the disease. In this work, we constructed a multi-signal sensing platform based on silicon nanoparticles (Si NPs) for β-Gal activity determination. When β-Gal was introduced to the sensing system, 2-Nitrophenyl β-D-galactopyranoside (ONPG) could be converted to o-Nitrophenol (o-NP), which had a characteristic absorption peak at 416 nm and the colorless solution turned yellow. The fluorescence emission of Si NPs at 450 nm can be greatly quenched by o-NP as a consequence of inner filter effect (IFE). This dual-signal fluorometric and colorimetric determination approach could be utilized to detect β-Gal in the range of 2-120 U/L and 6-120 U/L. The limits of detection were 1.36 U/L and 1.07 U/L, respectively. This sensing platform could be successfully utilized to detect β-Gal in real samples. Additionally, a visual detection method was designed to achieve quantitative analysis of β-Gal with the assistance of the smartphone.
作为早期卵巢癌的主要生物标志物之一，β-半乳糖苷酶（β-Gal）在疾病的发现和诊断中具有重要意义。在这项工作中，我们构建了一种基于硅纳米粒子（Si NPs）的多信号传感平台，用于β-Gal 活性测定。当β-Gal 被引入传感系统时，2-硝基苯-β-D-半乳糖苷（ONPG）可以转化为邻硝基苯酚（o-NP），其在 416nm 处具有特征吸收峰，无色溶液变为黄色。由于内滤效应（IFE），Si NPs 在 450nm 处的荧光发射可以被 o-NP 大大猝灭。这种双信号荧光和比色测定方法可以用于检测 2-120 U/L 和 6-120 U/L 范围内的β-Gal。检测限分别为 1.36 U/L 和 1.07 U/L。该传感平台可成功用于检测实际样品中的β-Gal。此外，设计了一种可视化检测方法，借助智能手机实现了β-Gal 的定量分析。
