International Center for Materials Nanoarchitectonics (WPI-MANA), National Institute for Materials Science (NIMS), Namiki 1-1, Tsukuba, Ibaraki 305-0044, Japan.
Research Network and Facility Services Division, National Institute for Materials Science (NIMS), 1-2-1 Sengen, Tsukuba, Ibaraki 305-0047, Japan.
J Am Chem Soc. 2022 Jun 22;144(24):10830-10843. doi: 10.1021/jacs.2c02596. Epub 2022 May 19.
Chromophores that generate singlet oxygen (O) in water are essential to developing noninvasive disease treatments using photodynamic therapy (PDT). A facile approach for formation of stable colloidal nanoparticles of O photosensitizers, which exhibit aggregation enhanced O generation in water toward applications as PDT agents, is reported. Chromophore encryption within a fuchsonarene macrocyclic scaffold insulates the photosensitizer from aggregation induced deactivation pathways, enabling a higher chromophore density than typical O generating nanoparticles. Aggregation enhanced O generation in water is observed, and variation in molecular structure allows for regulation of the physical properties of the nanoparticles which ultimately affects the O generation. activity and the ability of the particles to pass through the cell membrane into the cytoplasm is demonstrated using confocal fluorescence microscopy with HeLa cells. Photosensitizer encryption in rigid macrocycles, such as fuchsonarenes, offers new prospects for the production of biocompatible nanoarchitectures for applications involving O generation.
产生单线态氧 (O) 的发色团对于开发使用光动力疗法 (PDT) 的非侵入性疾病治疗方法至关重要。本文报道了一种简便的方法，可用于形成 O 敏化剂的稳定胶体纳米粒子，这些纳米粒子在水中表现出聚集增强的 O 生成，可作为 PDT 试剂应用。在富勒烯大环支架内对发色团进行加密，可使光敏剂免受聚集诱导失活途径的影响，从而能够实现比典型的 O 生成纳米粒子更高的发色团密度。在水中观察到聚集增强的 O 生成，并且分子结构的变化允许调节纳米粒子的物理性质，这最终会影响 O 生成。使用 HeLa 细胞的共聚焦荧光显微镜证明了活性以及粒子穿过细胞膜进入细胞质的能力。在富勒烯等刚性大环中对光敏剂进行加密，为涉及 O 生成的生物相容性纳米结构的生产提供了新的前景。
