Li Meng, Sun Jin-Hu, Meng Jian
Department of Stomatology, Central Hospital of Xuzhou, The Xuzhou Clinical College of Xuzhou Medical University. Xuzhou 221000, China. E-mail:
Shanghai Kou Qiang Yi Xue. 2022 Feb;31(1):32-37.
To investigate the anticancer effect of artesunate(ART) on human tongue squamous cell carcinoma (CAL27) cells and its possible mechanism.
CAL27 cells was pretreated with different doses of ART. Then, CCK-8 and colony forming methods were used for cell viability analysis, cell apoptosis was detected by flow cytometry, and cell migration and invasion capacity were determined by scratch test and Transwell chamber method. In addition, the expression of matrix metalloproteinase-9 (MMP-9) and vascular endothelial growth factor (VEGF) mRNA was measured by qPCR, and Western blot was used to detect the expression of MMP-9 and VEGF and activation of STAT3 signal in CAL27 cells treated with ART at various concentrations. SPSS 19.0 software package was used for statistical analysis of the data.
Artesunate significantly inhibited proliferation(P＜0.01), invasion(P＜0.01)and migration(P＜0.01) of CAL27 cells，and induced apoptosis of CAL27 cells (P＜0.01) in a dose-dependent manner. ART not only significantly reduced the expression of MMP-9 and VEGF mRNA in CAL27 cells in a dose-dependent manner(P＜0.05), but also inhibited the protein expression of p-STAT3, MMP-9 and VEGF.
Artesunate can inhibit the proliferation, migration and invasion of CAL27 cells, which may exert antitumor effects by inhibiting the STAT3 signaling pathway.
探讨青蒿琥酯（ART）对人舌鳞状细胞癌（CAL27）细胞的抗癌作用及其可能机制。
用不同剂量的ART预处理CAL27细胞。然后，采用CCK-8法和集落形成法进行细胞活力分析，通过流式细胞术检测细胞凋亡，采用划痕试验和Transwell小室法测定细胞迁移和侵袭能力。此外，用qPCR检测基质金属蛋白酶-9（MMP-9）和血管内皮生长因子（VEGF）mRNA的表达，用蛋白质免疫印迹法检测不同浓度ART处理的CAL27细胞中MMP-9和VEGF的表达及STAT3信号的激活情况。数据采用SPSS 19.0软件包进行统计分析。
青蒿琥酯能显著抑制CAL27细胞的增殖（P＜0.01）、侵袭（P＜0.01）和迁移（P＜0.01），并呈剂量依赖性诱导CAL27细胞凋亡（P＜0.01）。ART不仅能呈剂量依赖性显著降低CAL27细胞中MMP-9和VEGF mRNA的表达（P＜0.05），还能抑制p-STAT3、MMP-9和VEGF的蛋白表达。
青蒿琥酯可抑制CAL27细胞的增殖、迁移和侵袭，其可能通过抑制STAT3信号通路发挥抗肿瘤作用。
