斑蝥酸钠对人舌鳞状细胞癌CAL27细胞的抑制作用及机制

[Inhibitory effect and mechanism of sodium cantharidate on human tongue squamous cell carcinoma CAL27 cells].

作者信息

Li Xin-Ran, Chen Lin, Meng Jian

机构信息

Xuzhou Clinical College, Xuzhou Medical University, Xuzhou 221000, China. E-mail:

出版信息

Shanghai Kou Qiang Yi Xue. 2024 Jun;33(3):229-234.

PMID:39104334

Abstract

PURPOSE

To investigate the inhibitory effect of sodium cantharidate (SCA) on human tongue squamous cell carcinoma CAL27 cells and its mechanism.

METHODS

CAL27 cells were pretreated with different concentrations of SCA. Cell viability was analyzed by CCK-8 method. The migration and invasion of CAL27 cells were measured by scratch test and Transwell chamber, and the apoptosis rate was measured by flow cytometry. p53 protein and its phosphorylation sites Ser33, Ser37, Ser46, expression of BCL-2, BAX, and cleaved caspase 3 in CAL27 cells were detected by Western blot. Statistical analysis was performed with Graphpad Prism 9.0 software package.

RESULTS

Compared with the blank control group, the proliferation, migration and invasion of CAL27 cells in sodium cantharidate group were significantly decreased, and the apoptosis rate was significantly increased(P＜0.01) in a dose-dependent manner. The expression of p53 protein and its phosphorylation sites Ser33, Ser37, Ser46 protein was significantly up-regulated(P＜0.05 or P＜0.01). The expression of BCL-2 protein was down-regulated and the expression of BAX protein was significantly up-regulated(P＜0.05 or P＜0.01). The ratio of BCL-2/BAX was significantly decreased and the expression of cleaved caspase 3 protein was significantly up-regulated(P＜0.05 or P＜0.01).

CONCLUSIONS

SCA can inhibit the proliferation, migration and invasion of human tongue squamous cell carcinoma CAL27 cells. It also down-regulates the ratio of BCL-2/BAX and up-regulates the expression of cleaved caspase 3 protein by regulating the phosphorylation of p53 protein, which induces apoptosis.

摘要

目的

探讨斑蝥酸钠（SCA）对人舌鳞状细胞癌CAL27细胞的抑制作用及其机制。

方法

用不同浓度的SCA预处理CAL27细胞。采用CCK-8法分析细胞活力。通过划痕试验和Transwell小室检测CAL27细胞的迁移和侵袭能力，采用流式细胞术检测细胞凋亡率。通过蛋白质免疫印迹法检测CAL27细胞中p53蛋白及其磷酸化位点Ser33、Ser37、Ser46、BCL-2、BAX和裂解的caspase 3的表达。使用Graphpad Prism 9.0软件包进行统计分析。

结果

与空白对照组相比，斑蝥酸钠组CAL27细胞的增殖、迁移和侵袭能力明显降低，细胞凋亡率明显升高（P＜0.01），呈剂量依赖性。p53蛋白及其磷酸化位点Ser33、Ser37、Ser46蛋白的表达明显上调（P＜0.05或P＜0.01）。BCL-2蛋白表达下调，BAX蛋白表达明显上调（P＜0.05或P＜0.01）。BCL-2/BAX比值明显降低，裂解的caspase 3蛋白表达明显上调（P＜0.05或P＜0.01）。