Laboratory of Molecular Regulation of Neurogenesis, GIGA-Stem Cells and GIGA-Neurosciences, Interdisciplinary Cluster for Applied Genoproteomics (GIGA-R), University of Liège, CHU Sart Tilman, 4000 Liège, Belgium.
Laboratory of Mass Spectrometry, MolSys Research Unit, Liege University, Liege, Belgium.
Science. 2022 May 20;376(6595):eabn6204. doi: 10.1126/science.abn6204.
In the forebrain, ventrally derived oligodendrocyte precursor cells (vOPCs) travel tangentially toward the cortex together with cortical interneurons. Here, we tested in the mouse whether these populations interact during embryogenesis while migrating. By coupling histological analysis of genetic models with live imaging, we show that although they are both attracted by the chemokine Cxcl12, vOPCs and cortical interneurons occupy mutually exclusive forebrain territories enriched in this chemokine. Moreover, first-wave vOPC depletion selectively disrupts the migration and distribution of cortical interneurons. At the cellular level, we found that by promoting unidirectional contact repulsion, first-wave vOPCs steered the migration of cortical interneurons away from the blood vessels to which they were both attracted, thereby allowing interneurons to reach their proper cortical territories.
在前脑，腹侧衍生的少突胶质前体细胞（vOPC）与皮质中间神经元一起向皮质方向进行切线迁移。在这里，我们在小鼠中测试了这些群体在迁移过程中是否在胚胎发生时相互作用。通过将遗传模型的组织学分析与活体成像相结合，我们表明，尽管它们都被趋化因子 Cxcl12 吸引，但 vOPC 和皮质中间神经元占据了富含这种趋化因子的相互排斥的前脑区域。此外，第一波 vOPC 的耗竭选择性地破坏了皮质中间神经元的迁移和分布。在细胞水平上，我们发现，通过促进单向接触排斥，第一波 vOPC 引导皮质中间神经元的迁移远离它们都被吸引的血管，从而使中间神经元能够到达它们适当的皮质区域。
