Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an, China.
Department of Human Anatomy, Shaanxi University of Chinese Medicine, Xi'an, China.
Brain Behav. 2022 Jun;12(6):e2615. doi: 10.1002/brb3.2615. Epub 2022 May 19.
The central nucleus of the amygdala (CeA) has dense downward fiber projections towards the nucleus tractus solitary (NTS) and can modulate the activity of NTS taste neurons. However, whether CeA affects the nongenomic role of aldosterone (ALD) in regulating sodium intake at the NTS level remains unclear.
First, 40 adult male Sprague Dawley rats were divided into five groups, referring to different concentrations of ALD, to observe the sodium intake pattern compared with the vehicle (n = 8). ALD, the mineralocorticoid receptor antagonist spironolactone (SPI), and ALD + SPI were injected into the NTS. Then, the rats were divided into four groups (n = 16): bilateral/unilateral CeA electrolytic lesions, bilateral/unilateral CeA sham lesions. After recovery, one stainless steel 23-gauge cannula with two tubes was implanted into the rat NTS, and all rats underwent a recovery period of 7 days. Then, each group was divided into two subgroups that received aldosterone or control solution injection, and the cumulative intake of 0.3 mol/L NaCl solution was recorded within 30 min.
Bilateral CeA lesion eliminated the increased 0.3 mol/L NaCl intake induced by aldosterone microinjected into the NTS (CeA lesion: 0.3 ± 0.04 ml/30 min vs. sham lesion: 1.3 ± 0.3 ml/30 min). Unilateral CeA lesion reduced the increased NaCl intake induced by aldosterone microinjected into the NTS compared with the control group (p < .05) in the first 15 min but not in 15-30 min (p > .05). In sham lesion rats, aldosterone (5 ng/0.1 μl) still induced a significant increase in NaCl intake (aldosterone: 1.3 ± 0.3 ml/30 min vs. control: 0.25 ± 0.02 ml/30 min) (p < .05).
The results verified that the complete CeA may play an important role in aldosterone to regulate the nongenomic effect on rapid sodium intake.
杏仁中央核（CeA）有密集的向下纤维投射到孤束核（NTS），并可以调节 NTS 味觉神经元的活动。然而，CeA 是否影响醛固酮（ALD）在 NTS 水平调节钠摄入的非基因组作用尚不清楚。
首先，将 40 只成年雄性 Sprague Dawley 大鼠分为 5 组，分别给予不同浓度的 ALD，与载体组（n=8）进行比较，观察钠摄入模式。将 ALD、盐皮质激素受体拮抗剂螺内酯（SPI）和 ALD+SPI 注射到 NTS。然后，大鼠分为 4 组（n=16）：双侧/单侧 CeA 电解损毁，双侧/单侧 CeA 假损毁。恢复后，将一根带有两个管的不锈钢 23 号套管植入大鼠 NTS，所有大鼠均进行 7 天的恢复期。然后，每组再分为两个亚组，分别接受 ALD 或对照溶液注射，记录 30 分钟内 0.3mol/L NaCl 溶液的累积摄入量。
双侧 CeA 损毁消除了 ALD 微注射到 NTS 引起的 0.3mol/L NaCl 摄入量增加（CeA 损毁：0.3±0.04ml/30min vs. 假损毁：1.3±0.3ml/30min）。单侧 CeA 损毁减少了 ALD 微注射到 NTS 引起的 NaCl 摄入量增加，与对照组相比（p<.05）在第 15 分钟内，但在 15-30 分钟内没有（p>.05）。在假损毁大鼠中，ALD（5ng/0.1μl）仍引起 NaCl 摄入量的显著增加（ALD：1.3±0.3ml/30min vs. 对照：0.25±0.02ml/30min）（p<.05）。
结果验证了完整的 CeA 可能在 ALD 调节快速钠摄入的非基因组作用中发挥重要作用。
