State Key Lab of Bioelectronics, National Demonstration Center for Experimental Biomedical Engineering Education, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
Department of General Surgery, Affiliated Zhong-Da Hospital, Southeast University, Dingjiaqiao 87, Nanjing 210009, China.
ACS Biomater Sci Eng. 2022 Jun 13;8(6):2428-2436. doi: 10.1021/acsbiomaterials.2c00381. Epub 2022 May 19.
Common poly(lactide--glycolide) (-PLGA) has emerged as a biodegradable and biocompatible material in tissue engineering. However, the poor hydrophilicity and elasticity of -PLGA lead to its limited application in tissue engineering. To this end, an amphiphilic crosslinked four-armed poly(lactic--glycolide) was prepared. First, four-armed PLGA (4A-PLGA) was synthesized by polymerizing l-lactide (LA) and glycolide (GA) with pentaerythritol as the initiator. Then, the hydrophilic polymer poly(glutamate propylene ester) (PGPE) was prepared through the esterification of glutamic acid and 1,2-propanediol. The hydrophilic 4A-PLGA-PGPE was finally synthesized through the condensation reaction of 4A-PLGA and PGPE with the aid of triphosgene. 4A-PLGA-PGPE was then used to prepare amphiphilic membranes by electrospinning. It was demonstrated that the mechanical properties and biocompatibility of 4A-PLGA were improved after the introduction of PGPE. Furthermore, the introduction of glutamate improved the hydrophilicity of 4A-PLGA, thus effectively promoting cell entry and adhesion, which makes the electrospun 4A-PLGA-PGPE membranes promising for tissue engineering.
在组织工程中，常见的聚（丙交酯-乙交酯）（-PLGA）已成为一种可生物降解和生物相容的材料。然而，-PLGA 的亲水性和弹性差，限制了其在组织工程中的应用。为此，制备了一种两亲性交联的四臂聚（丙交酯-乙交酯）。首先，通过以季戊四醇为引发剂聚合 L-丙交酯（LA）和乙交酯（GA）合成四臂聚丙交酯（4A-PLGA）。然后，通过谷氨酸和 1,2-丙二醇的酯化反应制备亲水性聚合物聚（谷氨酸丙酯）（PGPE）。最后，在三光气的协助下，通过 4A-PLGA 和 PGPE 的缩合反应合成亲水性 4A-PLGA-PGPE。然后通过静电纺丝制备两亲性膜。结果表明，PGPE 的引入改善了 4A-PLGA 的力学性能和生物相容性。此外，谷氨酸的引入提高了 4A-PLGA 的亲水性，从而有效促进了细胞进入和黏附，这使得静电纺丝 4A-PLGA-PGPE 膜在组织工程中具有广阔的应用前景。
