Department of Biochemistry, Punjab Agricultural University, Ludhiana, 141004, India.
Environ Sci Pollut Res Int. 2022 Oct;29(47):70862-70881. doi: 10.1007/s11356-022-20762-5. Epub 2022 May 19.
A green house experiment was conducted to evaluate the efficacy of soil application of selenium (Se) in modulating metabolic changes in rice under arsenic (As) stress. Rice plants were grown over soil amended with sodium arsenate (25, 50 and 100 μM kg soil) with or without sodium selenate @ 0.5 and 1 mg kg soil in a complete randomized experimental design, and photosynthetic efficiency, nutrient uptake and nitrogen metabolism in rice leaves were estimated at tillering and grain filling stages. Se treatments significantly improved the toxic effects of As on plant height, leaf dry weight and grain yield. Arsenate treatment reduced uptake of Na, Mg, P, K, Ca, Mn, Fe and Zn and lowered chlorophyll, carotenoids and activities of enzymes of nitrogen metabolism (nitrate reductase, nitrite reductase, glutamine synthase and glutamate synthase) in rice leaves at both the stages in a dose-dependent fashion. Se application along with As improved photosynthesis, nutrient uptake and arsenate-induced effects on activities of enzymes of nitrogen metabolism with maximum impact shown by As50 + Se1 combination. Application of Se can modulate photosynthetic efficiency, nutrient uptake and alterations in nitrogen metabolism in rice Cv PR126 due to As stress that helped plants to adapt to excess As and resulted in improved plant growth.
进行了一项温室实验，以评估土壤施用硒（Se）在调节砷（As）胁迫下水稻代谢变化方面的功效。采用完全随机试验设计，在添加亚砷酸钠（25、50 和 100 μM kg 土壤）的土壤上种植水稻植株，并添加或不添加 0.5 和 1 mg kg 土壤的硒酸钠，在分蘖和灌浆期评估水稻叶片的光合作用效率、养分吸收和氮代谢。硒处理显著改善了砷对株高、叶片干重和籽粒产量的毒性作用。砷酸盐处理以剂量依赖的方式降低了水稻叶片中 Na、Mg、P、K、Ca、Mn、Fe 和 Zn 的吸收，并降低了叶绿素、类胡萝卜素和氮代谢酶（硝酸还原酶、亚硝酸还原酶、谷氨酰胺合成酶和谷氨酸合成酶）的活性。在两个阶段，硒的应用与砷一起提高了光合作用、养分吸收以及砷酸盐对氮代谢酶活性的影响，其中以 As50+Se1 组合的影响最大。由于砷胁迫，硒的应用可以调节水稻 Cv PR126 的光合作用效率、养分吸收和氮代谢的变化，这有助于植物适应过量的砷，从而促进植物生长。
