Huang Huimin, Fan Li, Zhao Yunlin, Jin Qi, Yang Guiyan, Zhao Di, Xu Zhenggang
Hunan Research Center of Engineering Technology for Utilization of Environmental and Resources Plant, Central South University of Forestry and Technology, Changsha, China.
Changsha Environmental Protection College, Changsha, China.
Front Microbiol. 2022 May 3;13:871581. doi: 10.3389/fmicb.2022.871581. eCollection 2022.
Heavy metal resistant bacteria play an important role in the metal biogeochemical cycle in soil, but the benefits of microbial oxidation for plants and soil have not been well-documented. The purpose of this study was to explore the contribution of two spp. to alleviate the antimony (Sb) toxicity in plants, and, then, to propose a bioremediation method for Sb contaminated soil, which is characterized by environmental protection, high efficiency, and low cost. This study explored the effects of HM5 and HM7 inoculation on and soil were evaluated under controlled Sb stressed conditions (0 and 100 mmol/L, antimony slag) through a pot experiment. The results show that the total root length, root volume, tips, forks, crossings, and root activities of with inoculation are higher than those of the control group, and the strains promote the plant absorption of Sb from the soil environment. Especially in the antimony slag treatment group, HM5 had the most significant effect on root promotion and promoting the absorption of Sb by . Compared with the control group, the total root length, root volume, tips, forks, crossings, and root activities increased by 64.54, 70.06, 70.04, 78.15, 97.73, and 12.95%, respectively. The absorption of Sb by root, stem, and leaf increased by 265.12, 250.00, and 211.54%, compared with the control group, respectively. Besides, both HM5 and HM7 reduce the content of malondialdehyde, proline, and soluble sugars in plant leaves, keeping the antioxidant enzyme activity of at a low level, and alleviating lipid peroxidation. Principal component analysis (PCA) shows that both HM5 and HM7 are beneficial to the maintenance of plant root functions and the improvement of the soil environment, thereby alleviating the toxicity of Sb. Therefore, HM5 and HM7 in phytoremediation with is a promising inoculant used for bacteria-assisted phytoremediation on Sb contaminated sites.
抗重金属细菌在土壤中的金属生物地球化学循环中发挥着重要作用，但微生物氧化对植物和土壤的益处尚未得到充分记录。本研究的目的是探讨两种菌株对减轻植物体内锑（Sb）毒性的贡献，进而提出一种用于锑污染土壤的生物修复方法，其特点是环保、高效且低成本。本研究通过盆栽试验，在受控的锑胁迫条件（0和100 mmol/L，锑渣）下，探究了接种HM5和HM7对植物和土壤的影响。结果表明，接种后的植物总根长、根体积、根尖数、分叉数、交叉数和根系活力均高于对照组，且这些菌株促进了植物从土壤环境中吸收锑。特别是在锑渣处理组中，HM5对根系促进和促进植物吸收锑的效果最为显著。与对照组相比，总根长、根体积、根尖数、分叉数、交叉数和根系活力分别增加了64.54%、70.06%、70.04%、78.15%、97.73%和12.95%。与对照组相比，根、茎和叶对锑的吸收分别增加了265.12%、250.00%和211.54%。此外，HM5和HM7均降低了植物叶片中丙二醛、脯氨酸和可溶性糖的含量，使植物的抗氧化酶活性保持在较低水平，减轻了脂质过氧化。主成分分析（PCA）表明，HM5和HM7均有利于维持植物根系功能和改善土壤环境，从而减轻锑的毒性。因此，在植物修复中使用HM5和HM7是一种有前途的接种剂，可用于锑污染场地的细菌辅助植物修复。
