sp. nov. and sp. nov., isolated from the leaves and rhizosphere soil of .

Two novel actinobacteria, designated strains GY16 and T44, were isolated from the leaves and rhizosphere soil of , respectively. A polyphasic approach was used for determining their taxonomic position. Results of 16S rRNA gene sequence analysis indicated that strain GY16 exhibited highest similarities to subsp. CGMCC 4.1593 (98.82 %), KCTC 19241 (98.76 %), NRRL B-16523 (98.69 %), KACC 20186 (98.69 %) and NBRC 13407 (98.69 %), and strain T44 showed 99.2, 99.1, 99.1 and <98.7 % sequence similarities to CGMCC 4.1452, subsp. DSM 40028, DSM 40539 and other species, respectively. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain GY16 formed an independent subclade, which indicated that strain GY16 should belong to a potential novel species; and strain T44 was closely related to CGMCC 4.1452, subsp. DSM 40028, DSM 40539 and DSM 40224. However, the multilocus sequence analysis evolutionary distance, average nucleotide identity and DNA-DNA hybridization values between closely related relatives were far from the species-level thresholds. In addition, phenotypic and chemotaxonomic characteristics further confirmed that strains GY16 and T44 belonged to two distinct species. Based on these results, it is concluded that the isolated strains represent novel species within the genus , for which the names sp. nov. (type strain GY16=CICC 24807=KCTC 49326) and sp. nov. (type strain T44=CICC 24819=JCM 33918) are proposed.