Ruinelli Michela, Blom Jochen, Smits Theo H M, Pothier Joël F
Environmental Genomics and Systems Biology Research Group, Institute for Natural Resources Sciences, Zurich University of Applied Sciences (ZHAW), Wädenswil, Switzerland.
Bioinformatics and Systems Biology, Justus-Liebig-University Giessen, Giessen, Germany.
Front Microbiol. 2022 May 3;13:804681. doi: 10.3389/fmicb.2022.804681. eCollection 2022.
Members of the species complex cause symptoms that are ranging from leaf spots to cankers on a multitude of plant species, including some of the genus . To date, a total of two species of the species complex and six different pathovars have been associated with diseases on spp., which were shown to belong to different phylogenetic units (phylogroups, PG) based on sequence similarity of housekeeping genes or whole genomes, suggesting that virulence to spp. may be the result of convergent pathoadaptation. In this study, a comparative genomics approach was used to determine genes significantly associated with strains isolated from spp. across a phylogeny of 97 strains belonging to the species complex. Our study revealed the presence of a set of orthologous proteins which were significantly associated with strains isolated from spp. than in strains isolated from other hosts or from non-agricultural environments. Among them, the type III effector HopAY predicted to encode for a C58 cysteine protease was found to be highly associated with strains isolated from spp. and revealed patterns supporting co-evolution and host adaptation.
该物种复合体的成员会引发多种症状，从叶斑病到多种植物物种上的溃疡病，包括该属的一些植物。迄今为止，该物种复合体共有两个物种和六个不同的致病型与 spp. 上的病害有关，基于管家基因或全基因组的序列相似性，这些病害被证明属于不同的系统发育单元（系统发育群，PG），这表明对 spp. 的毒力可能是趋同路径适应的结果。在本研究中，采用了比较基因组学方法来确定与从 spp. 分离的菌株显著相关的基因，这些菌株来自属于该物种复合体的97个菌株的系统发育树。我们的研究揭示了一组直系同源蛋白的存在，这些蛋白与从 spp. 分离的菌株的相关性显著高于从其他宿主或非农业环境中分离的菌株。其中，预测编码C58半胱氨酸蛋白酶的III型效应子HopAY被发现与从 spp. 分离的菌株高度相关，并揭示了支持共同进化和宿主适应的模式。
