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用于（）中定量逆转录PCR的合适内参基因的鉴定。

Identification of suitable reference genes for quantitative reverse transcription PCR in ().

作者信息

Zhao Gangjun, Wang Meng, Gan Yaqin, Gong Hao, Li Junxing, Zheng Xiaoming, Liu Xiaoxi, Zhao Siying, Luo Jianning, Wu Haibin

机构信息

Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640 China.

Guangdong Key Laboratory for New Technology Research of Vegetables, Guangzhou, 510640 China.

出版信息

Physiol Mol Biol Plants. 2022 Apr;28(4):737-747. doi: 10.1007/s12298-022-01182-8. Epub 2022 May 3.

DOI:10.1007/s12298-022-01182-8
PMID:35592479
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9110621/
Abstract

UNLABELLED

Reverse transcription real-time quantitative PCR is widely used to quantify gene expression. Reference genes are usually used as internal controls to measure the target gene expression level. To date, there is no consensus on the use of systematically validated reference genes in different tissues of . This study evaluated the expression stability of 11 candidate reference genes in different tissues using five algorithms (BestKeeper, comparative delta-Ct method, GeNorm, NormFinder, and RefFinder). Protein phosphatase 2A was the most stable gene, while alpha Tubulin was the least stable. The relative expression of ethylene-related genes in different tissues was also analyzed to reveal their role in sex determination. This study provides the basis for using suitable reference genes to evaluate targeted gene expression.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1007/s12298-022-01182-8.

摘要

未标注

逆转录实时定量PCR被广泛用于定量基因表达。参考基因通常用作内部对照来测量靶基因表达水平。迄今为止，关于在不同组织中系统验证的参考基因的使用尚无共识。本研究使用五种算法（BestKeeper、比较ΔCt法、GeNorm、NormFinder和RefFinder）评估了11个候选参考基因在不同组织中的表达稳定性。蛋白磷酸酶2A是最稳定的基因，而α微管蛋白是最不稳定的基因。还分析了乙烯相关基因在不同组织中的相对表达，以揭示它们在性别决定中的作用。本研究为使用合适的参考基因评估靶向基因表达提供了依据。

补充信息

在线版本包含可在10.1007/s12298-022-01182-8获取的补充材料。