Rastogi D, Henle K J, Nagle W A, Moss A J, Neilan B A, Rastogi S P
Int J Hyperthermia. 1987 Jan-Feb;3(1):63-70. doi: 10.3109/02656738709140373.
We have tested the reported ability of procaine to inhibit the induction and the development of thermotolerance in Chinese hamster ovary cells. Thermotolerance was induced either by hyperthermia alone (10 min, 45 degrees C) or by combining hyperthermia and procaine (5 min, 45 degrees C + 10 mM procaine) with heating times adjusted to yield similar cell survival after the conditioning treatments. Both the kinetics of thermotolerance development in fresh medium without procaine and the magnitude of thermotolerance 6 h after heat conditioning were similar for the two treatment groups. Development of thermotolerance in the presence of procaine was tested by adding the drug at 5 or 10 mM to culture medium between, but not during two fractionated heat treatments. Thermotolerance development was observed even in the presence of 10 mM procaine, but only if cell survival was corrected for the 37 degrees C-procaine toxicity. Complete survival curves of cells incubated for 6 h at 37 degrees C in 7.5 mM procaine between heat conditioning and test heating showed a D0 that was only 35 per cent lower than that of thermotolerant controls. The data are consistent with the reported sensitization to heat killing by procaine, but show that thermotolerance induction and development were only minimally perturbed by procaine.
我们已经测试了普鲁卡因抑制中国仓鼠卵巢细胞热耐受诱导和发展的能力。热耐受可以通过单独热疗（10分钟，45摄氏度）或通过热疗与普鲁卡因联合（5分钟，45摄氏度 + 10 mM普鲁卡因）诱导，加热时间经过调整以使预处理后细胞存活率相似。两个处理组在无普鲁卡因的新鲜培养基中热耐受发展的动力学以及热预处理6小时后的热耐受程度均相似。通过在两次分次热疗之间而非热疗期间向培养基中添加5或10 mM的药物来测试在普鲁卡因存在下热耐受的发展。即使存在10 mM普鲁卡因，也观察到了热耐受的发展，但前提是要对37摄氏度时普鲁卡因的毒性进行细胞存活率校正。在热预处理和测试加热之间于7.5 mM普鲁卡因中在37摄氏度孵育6小时的细胞的完整存活曲线显示，其D0仅比热耐受对照低35%。这些数据与所报道的普鲁卡因对热杀伤的增敏作用一致，但表明热耐受的诱导和发展仅受到普鲁卡因的轻微干扰。
