College of Biological Science and Technology, Hubei Minzu University, Hubei, China.
College of Life Sciences, Northeast Forestry University, Harbin, China.
Bioengineered. 2022 May;13(5):12475-12488. doi: 10.1080/21655979.2022.2076009.
Root-related or endophytic microbes in halophytes play an important role in adaptation to extreme saline environments. However, there have been few comparisons of microbial distribution patterns in different tissues associated with halophytes. Here, we analyzed the bacterial communities and distribution patterns of the rhizospheres and tissue endosphere in two species ( and Bunge) using the 16S rRNA gene sequencing. The results showed that the bacterial abundance and diversity in the rhizosphere were significantly higher than that of endophytic, but lower than that of bulk soil. Microbial-diversity analysis showed that the dominant phyla of all samples were and , among which were extremely abundant in all the tissue endosphere. Heatmap and Linear discriminant analysis Effect Size (LEfSe) results showed that there were notable differences in microbial community composition related to plant compartments. Different networks based on plant compartments exhibited distinct topological features. Additionally, the bulk soil and rhizosphere networks were more complex and showed higher centrality and connectedness than the three endosphere networks. These results strongly suggested that plant compartments, and not species, affect microbiome composition.
盐生植物中的根系相关或内生微生物在适应极端盐环境方面发挥着重要作用。然而，对于与盐生植物相关的不同组织中微生物分布模式的比较研究还很少。在这里，我们使用 16S rRNA 基因测序分析了两种盐生植物（ 和 ）的根际和组织内生细菌群落及其分布模式。结果表明，根际的细菌丰度和多样性明显高于内生细菌，但低于土壤细菌。微生物多样性分析表明，所有样品的优势门均为 和 ，其中在所有组织内生中极为丰富。热图和线性判别分析效应量（LEfSe）结果表明，与植物区室相关的微生物群落组成存在显著差异。基于植物区室的不同网络显示出不同的拓扑特征。此外，与三种内生网络相比，土壤和根际网络更复杂，表现出更高的中心性和连通性。这些结果强烈表明，是植物区室而不是物种影响了微生物组的组成。
