Furlong S T, Leary J A, Costello C E, Dawidowicz E A
J Lipid Res. 1986 Nov;27(11):1182-9.
A polar lipid accounting for 12.5% of the total lipid nitrogen has been isolated from the protozoan Acanthamoeba castellanii. On the basis of thin-layer chromatography and mass spectral analysis, the lipid has been identified as diacylglyceryltrimethylhomoserine (DGTS). Fast atom bombardment (FAB) mass spectra of DGTS are reported for the first time and are compared to the FAB mass spectra of phosphatidylcholines and the electron ionization (EI) and field desorption (FD) mass spectra of DGTS. Gas-liquid chromatographic-mass spectrometric (GLC-MS) analysis of the acyl chain composition of this lipid has shown that 87.5% consists of cis-9-octadecenoic acid. Plasma membrane isolated from this organism has shown that labeled DGTS appears in the plasma membrane but is not enriched in this fraction. DGTS has been isolated previously only from a limited number of green plants and one species of fungus. Identification of this lipid in Acanthamoeba indicates that this lipid is distributed among a diverse group of lower eucaryotes.
从原生动物卡氏棘阿米巴中分离出一种占总脂质氮12.5%的极性脂质。基于薄层色谱和质谱分析，该脂质被鉴定为二酰基甘油三甲基高丝氨酸（DGTS）。首次报道了DGTS的快原子轰击（FAB）质谱，并将其与磷脂酰胆碱的FAB质谱以及DGTS的电子电离（EI）和场解吸（FD）质谱进行了比较。对该脂质的酰基链组成进行气液色谱-质谱（GLC-MS）分析表明，87.5%由顺式-9-十八碳烯酸组成。从该生物体分离的质膜显示，标记的DGTS出现在质膜中，但在该组分中未富集。DGTS此前仅从有限数量的绿色植物和一种真菌中分离得到。在棘阿米巴中鉴定出这种脂质表明该脂质分布于多种低等真核生物中。
