Janero D R, Barrnett R
J Lipid Res. 1982 Feb;23(2):307-16.
A polar diacylglycerolipid isolated from the phototrophic green alga Chlamydomonas reinhardtii 137+ (wild-type) displays chromatographic and chemical identity with the either-linked homoserine lipid 1(3),2-diacylglyceryl-(3)-O-4'(N,N,N-trimethyl)homoserine (DGTS) of the golden alga Ochromonas danica. Subcellularly, DGTS is a constituent of a fraction of thylakoid membranes purified from Chlamydomonas whole-cell homogenates. The proportion of DGTS in the photosynthetic lamellae is approximately 40% of the total found in the alga. Cellular and thylakoid-membrane DGTS both have an unsaturated:saturated ratio of about 1.8 and contain predominantly hexadecanoic and octadecanoic fatty acids. Quantitatively, the fatty acid complement of thylakoid DGTS is distinct from the cellular DGTS fatty acid profile. The results provide the first demonstrations that DGTS is a bona fide membrane lipid and, specifically, that ether-linked homoserine lipid is a component of the thylakoid membrane of a phototrophic green-plant cell.
从光合绿藻莱茵衣藻137 +（野生型）中分离出的一种极性二酰基甘油脂质，在色谱和化学性质上与金黄藻丹麦赭球藻的醚键连接的高丝氨酸脂质1(3),2 - 二酰基甘油基-(3)-O-4'(N,N,N-三甲基)高丝氨酸（DGTS）相同。在亚细胞水平上，DGTS是从衣藻全细胞匀浆中纯化得到的一部分类囊体膜的组成成分。在光合片层中，DGTS的比例约占该藻类中发现的总量的40%。细胞和类囊体膜中的DGTS的不饱和:饱和比例均约为1.8，且主要含有十六烷酸和十八烷酸脂肪酸。从数量上看，类囊体DGTS的脂肪酸组成与细胞DGTS的脂肪酸谱不同。这些结果首次证明DGTS是一种真正的膜脂，具体而言，醚键连接的高丝氨酸脂质是光合绿色植物细胞类囊体膜的一个组成成分。
