Watson E D, Stokes C R, Bourne F J
J Endocrinol. 1987 Mar;112(3):443-8. doi: 10.1677/joe.0.1120443.
The function of blood and uterine luminal neutrophils from ovariectomized mares treated with ovarian steroids was investigated 18 h after intrauterine infusion of 1 X 10(9) Streptococcus zooepidemicus. Random migration of blood neutrophils under agarose was reduced by treatment with progesterone compared with that of neutrophils from oestradiol-treated and control mares. In-vitro addition of progesterone to blood neutrophils from acyclic ponies also reduced migration. Uterine neutrophils did not migrate under agarose which was probably an effect of bacterial phagocytosis. Hormone treatment had little effect on phagocytosis of yeast blastospores by blood neutrophils. Phagocytosis by uterine neutrophils from oestradiol-treated and control mares was significantly better than that by blood neutrophils. In progesterone-treated mares, however, phagocytosis by uterine neutrophils was significantly lower than that in the other two treatment groups and was similar to that measured in blood neutrophils. The results indicate a marked effect of progesterone in reducing both migration of blood neutrophils and phagocytosis by uterine neutrophils.
在向子宫内注入1×10⁹兽疫链球菌18小时后，对用卵巢类固醇处理的去卵巢母马的血液和子宫腔中性粒细胞的功能进行了研究。与用雌二醇处理的母马和对照母马的中性粒细胞相比，用孕酮处理会降低琼脂糖下血液中性粒细胞的随机迁移。向处于非发情周期的小马的血液中性粒细胞中体外添加孕酮也会降低迁移。子宫中性粒细胞在琼脂糖下不会迁移，这可能是细菌吞噬作用的结果。激素处理对血液中性粒细胞吞噬酵母芽生孢子的影响很小。用雌二醇处理的母马和对照母马的子宫中性粒细胞的吞噬作用明显优于血液中性粒细胞。然而，在孕酮处理的母马中，子宫中性粒细胞的吞噬作用明显低于其他两个处理组，并且与血液中性粒细胞中测得的吞噬作用相似。结果表明，孕酮在降低血液中性粒细胞的迁移和子宫中性粒细胞的吞噬作用方面具有显著作用。
