Watson E D, Stokes C R, David J S, Bourne F J
J Reprod Fertil. 1987 Mar;79(2):531-7. doi: 10.1530/jrf.0.0790531.
The bactericidal and phagocytic activities of blood neutrophils suspended in uterine washings and the mobilization of neutrophils into the uterine lumen were studied in ovariectomized mares receiving oestradiol benzoate (N = 4), progesterone (N = 4) or oily vehicle (N = 4). Uterine lavage was performed sequentially up to 144 h after induction of endometritis by intrauterine infusion of glycogen (1%). There was no significant difference between the 3 groups in speed of mobilization of neutrophils into the uterus in the first 6 h after infusion but there were significantly more uterine luminal neutrophils in progesterone-treated than in oestradiol-treated mares by 24 h after infusion (P less than 0.01). Uterine washings collected from progesterone-treated mares at 0, 24 and 144 h were significantly worse at promoting bactericidal activity by neutrophils than washings from oestradiol-treated and control mares (P less than 0.001). In oestrogen-treated and control mares bactericidal activity had increased by 144 h but in progesterone-treated mares bactericidal activity remained low. Neither treatment nor time affected the ability of washings to opsonize yeast blastospores. Elevated concentrations of progesterone in plasma were therefore associated with decreased bactericidal activity of neutrophils suspended in uterine washings but the generation of C3b in washings did not appear to be affected by hormone treatment.
在接受苯甲酸雌二醇（N = 4）、孕酮（N = 4）或油性载体（N = 4）的去卵巢母马中，研究了悬浮于子宫冲洗液中的血液中性粒细胞的杀菌和吞噬活性，以及中性粒细胞向子宫腔的动员情况。通过子宫内注入1%糖原诱导子宫内膜炎后，连续进行子宫灌洗，直至144小时。输注后前6小时，三组中性粒细胞向子宫动员的速度无显著差异，但输注后24小时，孕酮处理的母马子宫腔内中性粒细胞明显多于雌二醇处理的母马（P < 0.01）。在0、24和144小时从孕酮处理的母马收集的子宫冲洗液，促进中性粒细胞杀菌活性的能力明显低于从雌二醇处理的母马和对照母马收集的冲洗液（P < 0.001）。在雌激素处理的母马和对照母马中，杀菌活性在144小时时有所增加，但在孕酮处理的母马中，杀菌活性仍然较低。处理和时间均未影响冲洗液调理酵母芽生孢子的能力。因此，血浆中孕酮浓度升高与悬浮于子宫冲洗液中的中性粒细胞杀菌活性降低有关，但冲洗液中C3b的产生似乎不受激素处理的影响。
