Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee Roorkee 247667, Uttarakhand, India.
Laboratory Animal Facility, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu 180001, Jammu and Kashmir, India.
ACS Appl Bio Mater. 2022 Jun 20;5(6):2726-2740. doi: 10.1021/acsabm.2c00200. Epub 2022 May 20.
Chronic wound healing is a major threat all over the world. There are currently a plethora of biomaterials-based wound dressings available for wound healing applications. In this study, a dual protein-based (silk fibroin and sericin) nanofibrous scaffold from a natural source ( silkworm cocoons) with antibacterial and antioxidative properties for wound healing was investigated. An electrospun layer-by-layer silk protein-based nanofibrous scaffold was fabricated with a top layer of hydrophobic silk fibroin protein blended with polyvinyl alcohol (PVA), a middle layer of waste protein silk sericin loaded with silver(I) sulfadiazine as an antibacterial agent, and a bottom layer using silk fibroin blended with polycaprolactone (PCL). The trilayered nanofibrous scaffold with a smooth and bead-free morphology demonstrated excellent wettability, slow in vitro degradation, controlled drug release, and potent antibacterial and antioxidant properties. In vitro, the scaffold also demonstrated excellent hemocompatibility and biocompatibility. Furthermore, in vivo wound contraction, histological, and micro-CT investigations show complete wound healing and the formation of new skin tissue in a male Balb/c mouse model treated with the scaffold. The antioxidant properties of the sericin protein and SSD-based triple-layered nanofibrous scaffold protect the wound from bacterial infection and improve wound healing in a mouse model. The current study develops a dual protein-based nanofibrous scaffold with antibacterial and antioxidant properties as a promising wound dressing material.
慢性伤口愈合是全世界面临的主要威胁。目前有大量基于生物材料的伤口敷料可用于伤口愈合应用。在这项研究中，我们研究了一种源自天然来源（蚕茧）的具有抗菌和抗氧化特性的双蛋白（丝素蛋白和丝胶）纳米纤维支架，可用于伤口愈合。通过层层静电纺丝技术制备了一种基于丝蛋白的纳米纤维支架，顶层为疏水性丝素蛋白与聚乙烯醇（PVA）共混，中层为负载磺胺嘧啶银的废弃蛋白丝胶作为抗菌剂，底层为丝素蛋白与聚己内酯（PCL）共混。具有光滑无珠形貌的三层纳米纤维支架具有优异的润湿性、缓慢的体外降解、药物控制释放以及强大的抗菌和抗氧化性能。体外实验还表明，该支架具有良好的血液相容性和生物相容性。此外，体内伤口收缩、组织学和 micro-CT 研究表明，在雄性 Balb/c 小鼠模型中，用支架处理后可完全愈合伤口并形成新的皮肤组织。丝胶蛋白和基于 SSD 的三层纳米纤维支架的抗氧化特性可防止伤口感染并促进小鼠模型中的伤口愈合。本研究开发了一种具有抗菌和抗氧化特性的双蛋白纳米纤维支架，作为一种有前途的伤口敷料材料。
