Biomaterial and Tissue Engineering Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati - 781039, Assam, India.
Department of Veterinary Surgery and Radiology, West Bengal University of Animal and Fishery Sciences, Kolkata - 700037, West Bengal, India.
Acta Biomater. 2017 Jan 15;48:157-174. doi: 10.1016/j.actbio.2016.10.019. Epub 2016 Oct 13.
Bombyx mori silk fibroin (BMSF) as biopolymer has been extensively explored in wound healing applications. However, limited study is available on the potential of silk fibroin (SF) from non-mulberry (Antheraea assama and Philosamia ricini) silk variety. Herein, we have developed non-mulberry SF (NMSF) based electrospun mats functionalized with epidermal growth factor (EGF) and ciprofloxacin HCl as potential wound dressing. The NMSF based mats exhibited essential properties of wound dressing like biocompatibility, high water retention capacity (440%), water vapor transmission rate (∼2330gmday), high elasticity (∼2.6MPa), sustained drug release and antibacterial activity. Functionalized NMSF mats enhanced the proliferation of human dermal fibroblasts and HaCaT cells in vitro as compared to non-functionalized mats (p⩽0.01) showing effective delivery of EGF. Extensive in vivo wound healing assesment demonstrated accelerated wound healing, enhanced re-epithelialization, highly vascularized granulation tissue and higher wound maturity as compared to BMSF based mats. NMSF mats treated wounds showed regulated deposition of mature elastin, collagen and reticulin fibers in the extracellular matrix of skin. Presence of skin appendages and isotropic collagen fibers in the regenerated skin also demonstrated scar-less healing and aesthetic wound repair.
A facile fabrication of a ready-to-use bioactive wound dressing capable of concomitantly accelerating the healing process as well as deposition of the extracellular matrix (ECM) to circumvent further scarring complicacies has become a focal point of research. In this backdrop, our present work is based on non-mulberry silk fibroin (NMSF) electrospun antibiotic loaded semi-occlusive mats, mimicking the ECM of skin in terms of morphology, topology, microporous structure and mechanical stiffness. Regulation of ECM deposition and isotropic orientation evinced the potential of the mat as an instructive platform for skin regeneration. The unique peptide motifs of NMSF assisted the augmented recruitment of fibroblast, keratinocytes and endothelial cells leading to accelerated wound healing. Early progression of mature granulation, faster re-epithelialization and angiogenesis in the wounds in in vivo rabbit model forwarded the blended nanofibrous mats of NMSF and PVA ferrying EGF, apt for scarless healing.
家蚕丝素蛋白（BMSF）作为生物聚合物，已在伤口愈合应用中得到广泛研究。然而，关于非桑蚕丝素蛋白（Antheraea assama 和 Philosamia ricini）在丝纤维中的潜在应用，研究有限。在此，我们开发了基于非桑蚕丝素蛋白（NMSF）的电纺膜，该膜具有表皮生长因子（EGF）和盐酸环丙沙星的功能，可作为潜在的伤口敷料。基于 NMSF 的基质表现出伤口敷料的基本特性，如生物相容性、高保水能力（440%）、水蒸气透过率（约 2330gmday）、高弹性（约 2.6MPa）、持续药物释放和抗菌活性。与非功能化基质相比，功能化的 NMSF 基质在体外显著促进了人真皮成纤维细胞和 HaCaT 细胞的增殖（p ⩽ 0.01），表明 EGF 的有效传递。广泛的体内伤口愈合评估表明，与基于 BMSF 的基质相比，可加速伤口愈合、增强上皮再形成、高度血管化肉芽组织和更高的伤口成熟度。NMSF 基质处理的伤口显示出成熟弹性蛋白、胶原蛋白和网状纤维在皮肤细胞外基质中的调节沉积。再生皮肤中存在皮肤附属物和各向同性胶原纤维也证明了无疤痕愈合和美学伤口修复。
制造一种易于使用的生物活性伤口敷料，能够同时加速愈合过程并沉积细胞外基质（ECM），以避免进一步的疤痕并发症，已成为研究的重点。在此背景下，我们目前的工作基于非桑蚕丝素蛋白（NMSF）电纺抗生素负载半封闭基质，在形态、拓扑、微孔结构和机械刚度方面模仿皮肤的 ECM。ECM 沉积的调节和各向同性取向证明了该基质作为皮肤再生指导平台的潜力。NMSF 的独特肽基序有助于成纤维细胞、角质形成细胞和内皮细胞的大量募集，从而加速伤口愈合。在体内兔模型中，伤口中成熟肉芽的早期进展、更快的上皮再形成和血管生成，促进了 NMSF 和 PVA 携带 EGF 的混合纳米纤维基质的融合，有利于无疤痕愈合。
