Vajda T, Szalkai K, Garai A
J Inorg Biochem. 1987 Jan;29(1):49-57. doi: 10.1016/0162-0134(87)80011-9.
The influence of Mn2+, Fe3+, Co2+, and Zn2+ ions on the extent of trypsinogen activation has been determined for several ion concentrations at pH 7.4 and 36.4 degrees C. For the Mn2+ ion also the autocatalytic rate constants have been detected. The effect of Ca2+ has been reinvestigated for comparison purposes. The apparent dissociation constants of KMn2+ = 0.01 (M) and KCa2+ = 0.02 (M) have been found for the given metal ion-trypsinogen complexes. For Co2+ ion, however, only a slight effect and for Fe3+ and Zn2+ ions no significant effect could be detected on trypsinogen activation. The investigated ions are of empty, open, and completed d subshells of electrons and they are different also in their ionic size. The differences in effects of the ions are discussed on the basis of these factors.
在pH 7.4和36.4摄氏度条件下，针对几种离子浓度测定了Mn2+、Fe3+、Co2+和Zn2+离子对胰蛋白酶原激活程度的影响。对于Mn2+离子，还检测到了自催化速率常数。为作比较，重新研究了Ca2+的作用。对于给定的金属离子 - 胰蛋白酶原复合物，已发现KMn2+ = 0.01（M）和KCa2+ = 0.02（M）的表观解离常数。然而，对于Co2+离子，在胰蛋白酶原激活方面仅检测到轻微影响，而对于Fe3+和Zn2+离子则未检测到显著影响。所研究的离子具有空的、开放的和满的d电子亚层，并且它们的离子大小也不同。基于这些因素讨论了离子效应的差异。
