Department of Microbiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt.
Medical Microbiology and Infection Control, VU University Medical Center, Amsterdam, The Netherlands.
BMC Vet Res. 2022 May 20;18(1):191. doi: 10.1186/s12917-022-03294-5.
The goal of this study was to investigate the prevalence of extended-spectrum β-lactamase production in Enterobacterales isolated from retail sheep meat in Zagazig, Egypt.
One hundred random samples of sheep meat were collected from different retail butcher shops (n = 5) in the city of Zagazig, Egypt. Bacterial isolates were identified by MALDI-TOF MS and screened for antibiotic susceptibility by disk diffusion; further genotypic characterization of β-lactamase-encoding genes was performed with Real-Time PCR. E. coli strains were phylotyped with the Clermont triplex PCR method.
Of the total of 101 bacterial isolates recovered from retail sheep meat samples, 93 were E. coli, six were Enterobacter cloacae and two were Proteus mirabilis. As many as 17% of these 100 samples showed ESBL phenotypes, all were E. coli. The bla genes were detected in seven isolates (six were bla and one was bla), three isolates harboured bla (all were bla), and two carried genes of the bla family (both were bla). Eight E. coli isolates expressed ESBL phenotype but no bla, bla or bla genes were detected by PCR. ESBL- positive E. coli isolates were nearly equally distributed over the commensal groups A/B1 and the virulent group D.
Nearly one in five sheep meat samples was contaminated with ESBL-E. coli. This further corroborates the potential role played by contaminated meat in the increasing resistance rates that have been reported worldwide.
本研究旨在调查埃及宰加济格零售羊肉中肠杆菌科分离株产超广谱β-内酰胺酶的流行情况。
从埃及宰加济格市的不同零售肉店（n=5）随机采集 100 份羊肉样本。采用 MALDI-TOF MS 对细菌分离株进行鉴定，并通过纸片扩散法筛选抗生素敏感性；进一步采用 Real-Time PCR 对β-内酰胺酶编码基因进行基因特征分析。采用 Clermont 三联 PCR 方法对大肠杆菌菌株进行分类。
从零售羊肉样本中总共分离出 101 株细菌，其中 93 株为大肠杆菌，6 株为阴沟肠杆菌，2 株为奇异变形杆菌。这些 100 个样本中，多达 17%表现出 ESBL 表型，全部为大肠杆菌。在 7 株菌中检测到 bla 基因（6 株为 blaCTX-M，1 株为 blaTEM），3 株菌携带 blaCTX-M 基因（全部为 blaCTX-M-15），2 株菌携带 bla 家族基因（均为 blaCTX-M-3）。8 株大肠杆菌分离株表现出 ESBL 表型，但 PCR 未检测到 blaCTX-M、blaTEM 或 blaCTX-M-1 基因。ESBL 阳性大肠杆菌分离株在共生组 A/B1 和毒力组 D 中分布较为均匀。
近五分之一的羊肉样本受到 ESBL-E. coli 的污染。这进一步证实了污染的肉类在全球范围内导致耐药率上升所起的潜在作用。
