Punia Ashish, Mancuso Andrew, Banerjee Probal, Yang Nan-Loh
Department of Chemistry and Center for Engineered Polymeric Materials, ‡Department of Chemistry and Center for Development Neuroscience, and §Department of Biology, College of Staten Island and the Graduate Center, The City University of New York, New York, New York 10314, United States.
Department of Chemistry and Center for Engineered Polymeric Materials, Department of Chemistry and Center for Development Neuroscience, and §Department of Biology, College of Staten Island and the Graduate Center, The City University of New York, New York, New York 10314, United States.
ACS Macro Lett. 2015 Apr 21;4(4):426-430. doi: 10.1021/acsmacrolett.5b00102. Epub 2015 Apr 1.
Amphiphilic acrylic copolymers with hexamethyleneamine and poly(ethylene glycol) side chains can show >100-fold selectivity toward over red blood cells. Homopolymer with cationic pendant amine groups is highly hemolytic and antibacterial. Incorporation of approximately 33 mol % of poly(ethylene glycol) methyl ether methacrylate (PEGMA) led to 1300 times reduction in hemolytic activity, while maintaining high levels of antibacterial activity. The hemolytic activity of these PEGylated copolymers depends on the overall content and spatial distribution of the PEGMA units. Higher activity against than was observed for this polymer system, likely due to hydrogen bonding ability of the PEG side chains with polysaccharide cell wall of the bacteria. Field emission scanning electron microscopy analysis confirmed the bacterial membrane rupture activity exerted by these copolymers, whereas time-kill studies revealed significantly different bactericidal kinetics toward the Gram-negative and the Gram-positive .
带有六亚甲基胺和聚乙二醇侧链的两亲性丙烯酸共聚物对（此处原文缺失一种物质，无法准确翻译）的选择性比对红细胞高100倍以上。带有阳离子侧基胺基的均聚物具有高度溶血和抗菌性。掺入约33摩尔%的聚乙二醇甲基醚甲基丙烯酸酯（PEGMA）可使溶血活性降低1300倍，同时保持高水平的抗菌活性。这些聚乙二醇化共聚物的溶血活性取决于PEGMA单元的总体含量和空间分布。该聚合物体系对（此处原文缺失一种物质，无法准确翻译）的活性高于对（此处原文缺失一种物质，无法准确翻译）的活性，这可能是由于PEG侧链与细菌多糖细胞壁的氢键结合能力所致。场发射扫描电子显微镜分析证实了这些共聚物对细菌膜的破裂活性，而时间杀灭研究表明对革兰氏阴性菌（此处原文缺失一种细菌名称，无法准确翻译）和革兰氏阳性菌（此处原文缺失一种细菌名称，无法准确翻译）的杀菌动力学有显著差异。
