Foley Casey D, Zhang Boyu, Alb Alina M, Trimpin Sarah, Grayson Scott M
Department of Chemistry, Wayne State University, 5101 Cass Avenue, Detroit, Michigan 48202, United States.
ACS Macro Lett. 2015 Jul 21;4(7):778-782. doi: 10.1021/acsmacrolett.5b00299. Epub 2015 Jul 7.
The power of ion mobility spectrometry-mass spectrometry (IMS-MS) as an analytical technology for differentiating macromolecular architecture is demonstrated. The presence of architectural dispersity within a sample is probed by sequentially measuring both the drift time and the mass-to-charge ratio for every component within a polymer sample. The utility of this technology is demonstrated by investigating three poly(ethylene glycol) (PEG) architectures with closely related average molecular weights of about 9000 Da: a linear PEG, an unevenly branched miktoarm star PEG, and evenly branched homoarm star PEGs. The three architectures were readily distinguished when analyzed separately as "pure" architectures or when analyzed as mixtures. IMS-MS results are contrasted with matrix-assisted laser desorption/ionization-MS and viscometry measurements.
离子淌度谱-质谱（IMS-MS）作为一种区分大分子结构的分析技术的能力得到了证明。通过依次测量聚合物样品中每个组分的漂移时间和质荷比，来探测样品中结构分散性的存在。通过研究三种平均分子量约为9000 Da且密切相关的聚乙二醇（PEG）结构：线性PEG、支化不均匀的多臂星型PEG和支化均匀的同臂星型PEG，证明了该技术的实用性。当作为“纯”结构单独分析或作为混合物分析时，这三种结构很容易区分。IMS-MS结果与基质辅助激光解吸/电离质谱和粘度测量结果形成对比。
