Department of Laboratory Medicine, Ningbo Medical Center, Lihuili Hospital, Ningbo University, Ningbo, China.
Department of Cardiothoracic Surgery, Ningbo Medical Center, Lihuili Hospital, Ningbo University, Ningbo, China.
J Clin Lab Anal. 2022 Aug;36(8):e24504. doi: 10.1002/jcla.24504. Epub 2022 May 21.
The purpose of this study was to explore the detection value of seven autoantibodies (TAAbs): p53, PGP9.5, SOX2, GBU4-5, MAGE A1, CAGE, and GAGE7 and three tumor markers: CYFRA21-1, NSE, and SCCA in the diagnosis of lung cancer.
ELISA was used to detect the levels of the TAAbs, and chemiluminescence immunoassay was used to test the levels of the tumor markers. The diagnostic efficacy of the TAAbs combined with the tumor markers for lung cancer was evaluated by receiver operating characteristic (ROC) curves.
The positive rate of the combined detection of seven TAAbs and three tumor markers in lung cancer (37.8%) was higher than that in other three groups. The positive rates of SOX2, GAGE7, MAGE A1, CAGE, CYFRA21-1, and SCCA had differences among the four groups. Compared with the benign lung disease group, only GAGE7, CYFRA21-1, and SCCA differed among the groups. The combined sensitivity of the TAAbs was 29.07% (AUC, 0.594), the combined sensitivity of all the markers was 37.76% (AUC, 0.660 [p < 0.05]), and Youden's index was 0.196. In the lung cancer group, CYFRA21-1 had a significant difference in age and sex, and SOX2, MAGE A1, CYFRA21-1, NSE, and SCCA were significantly different in pathological type and TNM. In contrast, p53 and GBU4-5 showed no significant differences in age, sex, pathological type, and TNM.
The combined detection of seven TAAbs and three tumor markers could be useful in early diagnosis of lung cancer.
本研究旨在探讨七种自身抗体（TAAbs）：p53、PGP9.5、SOX2、GBU4-5、MAGE A1、CAGE 和 GAGE7 以及三种肿瘤标志物：CYFRA21-1、NSE 和 SCCA 在肺癌诊断中的检测价值。
采用酶联免疫吸附试验（ELISA）检测 TAAbs 水平，化学发光免疫分析法检测肿瘤标志物水平。采用受试者工作特征（ROC）曲线评价 TAAbs 联合肿瘤标志物对肺癌的诊断效能。
七种 TAAbs 和三种肿瘤标志物联合检测肺癌的阳性率（37.8%）高于其他三组。SOX2、GBU4-5、MAGE A1、CAGE、CYFRA21-1 和 SCCA 的阳性率在四组之间存在差异。与良性肺部疾病组相比，仅 GAGE7、CYFRA21-1 和 SCCA 三组之间存在差异。TAAbs 的联合灵敏度为 29.07%（AUC，0.594），所有标志物的联合灵敏度为 37.76%（AUC，0.660[p<0.05]），Youden 指数为 0.196。在肺癌组中，CYFRA21-1 在年龄和性别方面存在显著差异，SOX2、MAGE A1、CYFRA21-1、NSE 和 SCCA 在病理类型和 TNM 分期方面存在显著差异。相比之下，p53 和 GBU4-5 在年龄、性别、病理类型和 TNM 分期方面无显著差异。
七种 TAAbs 和三种肿瘤标志物联合检测有助于肺癌的早期诊断。
