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拟南芥简化花浸转化方法。

Simplified floral dip transformation method of Arabidopsis thaliana.

机构信息

Centre for Plant Science and Biodiversity, University of Swat, Charbagh 19120, Pakistan; State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.

Centre for Plant Science and Biodiversity, University of Swat, Charbagh 19120, Pakistan.

出版信息

J Microbiol Methods. 2022 Jun;197:106492. doi: 10.1016/j.mimet.2022.106492. Epub 2022 May 19.

DOI:10.1016/j.mimet.2022.106492

PMID:35597520

Abstract

Being a model plant, Arabidopsis transformation is a frequent routine in genetics and molecular laboratories. Here, we explain a modified floral dip method routinely used in our laboratory for Arabidopsis transformation by applying drought stress after plant treatment with Agrobacterium. By using this method, hundreds of individual transgenic plants are produced from just 30 infiltrated plants by increasing transformation frequency from 1% to 10% within 2-3 months.

摘要

作为一种模式植物，拟南芥转化是遗传学和分子生物学实验室中的常见常规操作。在这里，我们介绍了一种经过改良的花浸法，该方法在我们实验室中常规用于对拟南芥进行干旱胁迫处理后的农杆菌转化。通过使用这种方法，我们可以在 2-3 个月内将转化频率从 1%提高到 10%，从而从 30 株浸染植株中产生数百株独立的转基因植株。