Chen Siyu, Zhang Lu, Ma Qianqian, Chen Meixiu, Cao Xiaolei, Zhao Sifeng, Zhang Xuekun
Key Laboratory at the Universities of Xinjiang Uygur Autonomous Region for Oasis Agricultural Pest Management and Plant Protection Resource Utilization, Agriculture College, Shihezi University, Shihezi 832003, China.
Plants (Basel). 2024 May 29;13(11):1493. doi: 10.3390/plants13111493.
Pers. is a holoparasitic plant that severely reduces tomato ( L.) production in China. However, there is a lack of effective control methods and few known sources of genetic resistance. In this study, we focused on key genes in the family, comparing the family in (L. Heynh.) to the tomato genome. After identifying the family members in , we performed chromosomal localization and linear analysis with phylogenetic relationship analysis of the family. We also analyzed the gene structure of the gene family members in tomato and the homology of the genes among the different species to study their relatedness. The key genes for resistance were identified using VIGS (virus-induced gene silencing), and the parasitization rate of silenced tomato plants against increased by 47.23-91.13%. The genes were localized in the nucleus by subcellular localization. Heterologous overexpression in showed that the key gene had a strong effect on the parasitization process of , and the overexpression of the key gene reduced the parasitization rate of 1.69-fold. Finally, it was found that the gene can positively regulate the hormone content in tomato plants and affect plant growth and development, further elucidating the function of this gene.
Pers.是一种全寄生植物，在中国严重降低番茄（L.）的产量。然而，缺乏有效的防治方法，且已知的抗源很少。在本研究中，我们聚焦于该家族中的关键基因，将（L. Heynh.）中的该家族与番茄基因组进行比较。在鉴定出中的该家族成员后，我们对该家族进行了染色体定位和线性分析，并进行了系统发育关系分析。我们还分析了番茄中该基因家族成员的基因结构以及不同物种间该基因的同源性，以研究它们的相关性。利用病毒诱导基因沉默（VIGS）鉴定出了抗Pers.的关键基因，沉默番茄植株对Pers.的寄生率提高了47.23 - 91.13%。通过亚细胞定位将这些基因定位在细胞核中。在中进行异源过表达表明，关键基因对Pers.的寄生过程有很强的影响，关键基因的过表达使Pers.的寄生率降低了1.69倍。最后发现，该基因可正向调节番茄植株中的激素含量并影响植物生长发育，进一步阐明了该基因的功能。
