Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan.
Industrial Microbiology, Graduate School of Agriculture, Kyoto University, Kitashirakawa Oiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan.
J Biosci Bioeng. 2022 Jul;134(1):84-88. doi: 10.1016/j.jbiosc.2022.04.009. Epub 2022 May 19.
Quantification of leuco-indigo is most important for Aizome, Japanese indigo-dyeing; however, there has been no convenient quantitative method. This study demonstrated that normal pulse voltammetry under quiescent conditions can be used to detect leuco-indigo. As a result of quantification of leuco-indigo in the depth direction in fermenting suspensions, the steady-state concentrations of leuco-indigo showed sigmoidal profiles in the depth direction. The steady state is caused by competitive reactions of microbial reduction of indigo and autoxidation of leuco-indigo by O dissolved from the air interface of the suspension. In addition, we investigated the effects of stirring the suspension and adding some nutrients to the concentration profile. The weakened activity was partially recovered by the addition of ethanol and remarkably recovered by the addition of hipolypepton or glucose. Knowledge is essential for the proper management of indigo-dye-fermenting suspensions.
靛白的定量对于日本蓝染的青黛染色最为重要；然而，一直没有方便的定量方法。本研究表明，在静止条件下进行常规脉冲伏安法可以检测到靛白。由于在发酵悬浮液中沿深度方向对靛白进行定量，因此在深度方向上，靛白的稳态浓度呈现出钟形曲线。这种稳定状态是由微生物还原靛蓝的竞争反应和溶解在悬浮液空气界面处的 O 自氧化引起的。此外，我们研究了搅拌悬浮液和向浓度分布中添加一些营养物质的影响。通过添加乙醇可以部分恢复减弱的活性，而通过添加低聚肽或葡萄糖可以显著恢复。了解这些知识对于靛蓝染料发酵悬浮液的正确管理至关重要。
