• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

mAm甲基转移酶PCIF1通过抑制TM9SF1 mRNA翻译对胃癌细胞的侵袭性至关重要。

mAm methyltransferase PCIF1 is essential for aggressiveness of gastric cancer cells by inhibiting TM9SF1 mRNA translation.

作者信息

Zhuo Wei, Sun Meng, Wang Kun, Zhang Lu, Li Kai, Yi Danyang, Li Mengjie, Sun Qiang, Ma Xixi, Liu Wei, Teng Lisong, Yi Chengqi, Zhou Tianhua

机构信息

Department of Cell Biology and Department of Gastroenterology of Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China.

State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, China.

出版信息

Cell Discov. 2022 May 21;8(1):48. doi: 10.1038/s41421-022-00395-1.

DOI:10.1038/s41421-022-00395-1
PMID:35597784
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9124189/
Abstract

PCIF1 (phosphorylated CTD interacting factor 1) is the first reported RNA N6,2'-O-dimethyladenosine (mAm) methyltransferase. However, the pathological significance of PCIF1 and mAm modification remains unknown. Here we find that both PCIF1 expression and mAm modification are significantly elevated in gastric cancer tissues. Increased PCIF1 is associated with gastric cancer progression, and predicts poor prognosis. Silence of PCIF1 inhibits the proliferation and invasion of gastric cancer cells, and suppresses tumor growth and metastasis in mouse model. mAm-seq analysis reveals TM9SF1 (transmembrane 9 superfamily member 1) as a target of PCIF1. PCIF1 modifies TM9SF1 mRNA with mAm leading to decreased TM9SF1 translation. TM9SF1 reverses the effects of PCIF1 on gastric cancer cell aggressiveness. Collectively, our work uncovers an oncogenic function of PCIF1, providing insights into the critical role of mAm modification in cancer progression.

摘要

PCIF1（磷酸化CTD相互作用因子1）是首个被报道的RNA N6,2'-O-二甲基腺苷（mAm）甲基转移酶。然而，PCIF1和mAm修饰的病理意义仍不清楚。在此我们发现，PCIF1的表达和mAm修饰在胃癌组织中均显著升高。PCIF1的增加与胃癌进展相关，并预示着预后不良。沉默PCIF1可抑制胃癌细胞的增殖和侵袭，并在小鼠模型中抑制肿瘤生长和转移。mAm序列分析显示TM9SF1（跨膜9超家族成员1）是PCIF1的一个靶点。PCIF1通过mAm修饰TM9SF1 mRNA，导致TM9SF1翻译减少。TM9SF1可逆转PCIF1对胃癌细胞侵袭性的影响。总体而言，我们的研究揭示了PCIF1的致癌功能，为mAm修饰在癌症进展中的关键作用提供了见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb73/9124189/6ea1af00c74a/41421_2022_395_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb73/9124189/8783a5f98d25/41421_2022_395_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb73/9124189/dc304e9f8932/41421_2022_395_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb73/9124189/82919c75667f/41421_2022_395_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb73/9124189/14a043d692cb/41421_2022_395_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb73/9124189/196cc27641dc/41421_2022_395_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb73/9124189/6ea1af00c74a/41421_2022_395_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb73/9124189/8783a5f98d25/41421_2022_395_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb73/9124189/dc304e9f8932/41421_2022_395_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb73/9124189/82919c75667f/41421_2022_395_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb73/9124189/14a043d692cb/41421_2022_395_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb73/9124189/196cc27641dc/41421_2022_395_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fb73/9124189/6ea1af00c74a/41421_2022_395_Fig6_HTML.jpg

相似文献

1
mAm methyltransferase PCIF1 is essential for aggressiveness of gastric cancer cells by inhibiting TM9SF1 mRNA translation.mAm甲基转移酶PCIF1通过抑制TM9SF1 mRNA翻译对胃癌细胞的侵袭性至关重要。
Cell Discov. 2022 May 21;8(1):48. doi: 10.1038/s41421-022-00395-1.
2
HIV reprograms host mAm RNA methylome by viral Vpr protein-mediated degradation of PCIF1.HIV 通过病毒 Vpr 蛋白介导的 PCIF1 降解重编程宿主 mAm RNA 甲基化组。
Nat Commun. 2021 Sep 20;12(1):5543. doi: 10.1038/s41467-021-25683-4.
3
mAm Methyltransferase PCIF1 Regulates Periodontal Inflammation.mAm 甲基转移酶 PCIF1 调控牙周炎炎症。
J Dent Res. 2024 Oct;103(11):1130-1140. doi: 10.1177/00220345241271078. Epub 2024 Sep 18.
4
Enzymatic characterization of mRNA cap adenosine-N6 methyltransferase PCIF1 activity on uncapped RNAs.鉴定无帽 mRNA 上 PCIF1 对帽结构腺苷-N6 甲基转移酶活性的酶学特征。
J Biol Chem. 2022 Apr;298(4):101751. doi: 10.1016/j.jbc.2022.101751. Epub 2022 Feb 19.
5
PCIF1, the only methyltransferase of N6,2-O-dimethyladenosine.PCIF1，N6,2'-O-二甲基腺苷的唯一甲基转移酶。
Cancer Cell Int. 2023 Oct 1;23(1):226. doi: 10.1186/s12935-023-03066-7.
6
A Pan-Cancer Analysis of the Oncogenic and Immunogenic Role of m6Am Methyltransferase PCIF1.m6Am甲基转移酶PCIF1致癌和免疫原性作用的泛癌分析
Front Oncol. 2021 Nov 23;11:753393. doi: 10.3389/fonc.2021.753393. eCollection 2021.
7
Identification of the mAm Methyltransferase PCIF1 Reveals the Location and Functions of mAm in the Transcriptome.鉴定 mAm 甲基转移酶 PCIF1 揭示了 mAm 在转录组中的位置和功能。
Mol Cell. 2019 Aug 8;75(3):631-643.e8. doi: 10.1016/j.molcel.2019.06.006. Epub 2019 Jul 3.
8
The Mammalian Cap-Specific mAm RNA Methyltransferase PCIF1 Regulates Transcript Levels in Mouse Tissues.哺乳动物帽特异性 mAm RNA 甲基转移酶 PCIF1 调节小鼠组织中的转录水平。
Cell Rep. 2020 Aug 18;32(7):108038. doi: 10.1016/j.celrep.2020.108038.
9
m6Am methyltransferase PCIF1 negatively regulates ciliation by inhibiting BICD2 expression.m6Am 甲基转移酶 PCIF1 通过抑制 BICD2 的表达来负调控纤毛形成。
J Cell Biol. 2024 Jun 3;223(6). doi: 10.1083/jcb.202307002. Epub 2024 Mar 25.
10
m6Am Methyltransferase PCIF1 Promotes LPP3 Mediated Phosphatidic Acid Metabolism and Renal Cell Carcinoma Progression.m6Am甲基转移酶PCIF1促进LPP3介导的磷脂酸代谢和肾细胞癌进展。
Adv Sci (Weinh). 2024 Dec;11(46):e2404033. doi: 10.1002/advs.202404033. Epub 2024 Oct 18.

引用本文的文献

1
SERBP1-PCIF1 complex-controlled m6Am modification in glutamatergic neurons of the primary somatosensory cortex is required for neuropathic pain in mice.原发性体感皮层谷氨酸能神经元中SERBP1-PCIF1复合物控制的m6Am修饰是小鼠神经性疼痛所必需的。
Nat Commun. 2025 Aug 5;16(1):7225. doi: 10.1038/s41467-025-62565-5.
2
RNA demethylase FTO uses conserved aromatic residues to recognize the mRNA 5' cap and promote efficient mA demethylation.RNA去甲基化酶FTO利用保守的芳香族残基识别mRNA 5'帽并促进高效的m⁶A去甲基化。
bioRxiv. 2025 May 10:2025.05.09.653100. doi: 10.1101/2025.05.09.653100.
3
Introduction of human mAm methyltransferase PCIF1 facilitates the biosynthesis of terpenoids in Saccharomyces cerevisiae.

本文引用的文献

1
Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries.《全球癌症统计数据 2020：全球 185 个国家和地区 36 种癌症的发病率和死亡率估计》。
CA Cancer J Clin. 2021 May;71(3):209-249. doi: 10.3322/caac.21660. Epub 2021 Feb 4.
2
Systematic profiling of alternative splicing signature reveals prognostic predictor for cervical cancer.系统分析可变剪接特征揭示宫颈癌的预后预测因子。
J Transl Med. 2019 Nov 19;17(1):379. doi: 10.1186/s12967-019-02140-x.
3
Landscape and Regulation of mA and mAm Methylome across Human and Mouse Tissues.
人源mAm甲基转移酶PCIF1的引入促进了酿酒酵母中萜类化合物的生物合成。
Microb Cell Fact. 2025 Apr 2;24(1):78. doi: 10.1186/s12934-025-02701-4.
4
TM9SF1 inhibits colorectal cancer metastasis by targeting Vimentin for Tollip-mediated selective autophagic degradation.TM9SF1通过靶向波形蛋白进行Tollip介导的选择性自噬降解来抑制结直肠癌转移。
Cell Death Differ. 2025 Apr 2. doi: 10.1038/s41418-025-01498-4.
5
PCIF1 drives oesophageal squamous cell carcinoma progression via m6Am-mediated suppression of MTF2 translation.PCIF1通过m6Am介导的MTF2翻译抑制驱动食管鳞状细胞癌进展。
Clin Transl Med. 2025 Apr;15(4):e70286. doi: 10.1002/ctm2.70286.
6
The epitranscriptional factor PCIF1 orchestrates CD8 T cell ferroptosis and activation to control antitumor immunity.表观转录因子PCIF1协调CD8 T细胞铁死亡和激活以控制抗肿瘤免疫。
Nat Immunol. 2025 Feb;26(2):252-264. doi: 10.1038/s41590-024-02047-w. Epub 2025 Jan 6.
7
Cap-specific terminal N6-methyladeonsine methylation of RNA mediated by PCIF1 and possible therapeutic implications.由PCIF1介导的RNA的帽特异性末端N6-甲基腺嘌呤甲基化及其潜在治疗意义。
Genes Dis. 2023 Nov 23;12(1):101181. doi: 10.1016/j.gendis.2023.101181. eCollection 2025 Jan.
8
Cap-Specific mAm Methyltransferase PCIF1/CAPAM Regulates mRNA Stability of and through the mA Methyltransferase Activity.帽特异性 mAm 甲基转移酶 PCIF1/CAPAM 通过 mA 甲基转移酶活性调控 和 的 mRNA 稳定性。
Cells. 2024 Oct 12;13(20):1689. doi: 10.3390/cells13201689.
9
m6Am Methyltransferase PCIF1 Promotes LPP3 Mediated Phosphatidic Acid Metabolism and Renal Cell Carcinoma Progression.m6Am甲基转移酶PCIF1促进LPP3介导的磷脂酸代谢和肾细胞癌进展。
Adv Sci (Weinh). 2024 Dec;11(46):e2404033. doi: 10.1002/advs.202404033. Epub 2024 Oct 18.
10
mAm Methyltransferase PCIF1 Regulates Periodontal Inflammation.mAm 甲基转移酶 PCIF1 调控牙周炎炎症。
J Dent Res. 2024 Oct;103(11):1130-1140. doi: 10.1177/00220345241271078. Epub 2024 Sep 18.
人类和小鼠组织中 mA 和 mAm 甲基组的景观和调控。
Mol Cell. 2020 Jan 16;77(2):426-440.e6. doi: 10.1016/j.molcel.2019.09.032. Epub 2019 Oct 29.
4
PCIF1 Catalyzes m6Am mRNA Methylation to Regulate Gene Expression.PCIF1 催化 m6Am mRNA 甲基化以调节基因表达。
Mol Cell. 2019 Aug 8;75(3):620-630.e9. doi: 10.1016/j.molcel.2019.05.030. Epub 2019 Jul 3.
5
Identification of the mAm Methyltransferase PCIF1 Reveals the Location and Functions of mAm in the Transcriptome.鉴定 mAm 甲基转移酶 PCIF1 揭示了 mAm 在转录组中的位置和功能。
Mol Cell. 2019 Aug 8;75(3):631-643.e8. doi: 10.1016/j.molcel.2019.06.006. Epub 2019 Jul 3.
6
Where, When, and How: Context-Dependent Functions of RNA Methylation Writers, Readers, and Erasers.在哪里、何时以及如何：RNA 甲基化写入器、读取器和擦除器的上下文相关功能。
Mol Cell. 2019 May 16;74(4):640-650. doi: 10.1016/j.molcel.2019.04.025.
7
Cap-specific, terminal N-methylation by a mammalian mAm methyltransferase.由哺乳动物的mAm甲基转移酶进行的帽特异性末端N-甲基化。
Cell Res. 2019 Jan;29(1):80-82. doi: 10.1038/s41422-018-0117-4. Epub 2018 Nov 28.
8
Cap-specific terminal -methylation of RNA by an RNA polymerase II-associated methyltransferase.RNA 聚合酶 II 相关甲基转移酶对 RNA 的帽特异性末端 - 甲基化。
Science. 2019 Jan 11;363(6423). doi: 10.1126/science.aav0080. Epub 2018 Nov 22.
9
Differential mA, mA, and mA Demethylation Mediated by FTO in the Cell Nucleus and Cytoplasm.FTO 在细胞核和细胞质中介导的差异 mA、mA 和 mA 去甲基化。
Mol Cell. 2018 Sep 20;71(6):973-985.e5. doi: 10.1016/j.molcel.2018.08.011. Epub 2018 Sep 6.
10
RNA N-methyladenosine modification in cancers: current status and perspectives.癌症中的 RNA N6-甲基腺苷修饰：现状与展望。
Cell Res. 2018 May;28(5):507-517. doi: 10.1038/s41422-018-0034-6. Epub 2018 Apr 23.