School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, 730050, China.
School of Petrochemical Engineering, Lanzhou University of Technology, Lanzhou, 730050, China.
J Plant Physiol. 2022 Jul;274:153718. doi: 10.1016/j.jplph.2022.153718. Epub 2022 May 13.
In this study, we focused on the plant-growth-promoting properties of two strains isolated from Codonopsis pilosula, and the effect of inoculation with different strain treatments on physiological and metabolite accumulation of C. pilosula. The strains RHBA19 and RHBA17 were isolated and identified as Geobacillu sp. and Pseudomonas fluorescens, respectively. The two strains produced indole acetic acid (IAA), siderophore, biofilm, and various exoenzymes. Based on the pot experiments, inoculation of RHBA19 (G group) and the two mixed bacteria (M group) significantly improved the growth, root development, and photosynthesis of C. pilosula. Compared with the no-inoculation group (CK), the total polysaccharide content of root in the G and M groups was dramatically enhanced by 59.27% and 96.07%, and the lobetyolin (root) improved by 58.4% and 66.0%, respectively. After inoculation with bacteria agents, the activities of antioxidant enzymes (CAT, POD, SOD) of C. pilosula increased differentially. Inoculation with two types of bacterial agents significantly increased the activities of sucrose synthase (SS) and sucrose phosphate synthase (SPS) in root, and phenylalanine ammonia lyase (PAL) in leaf of C. pilosula. In addition, the content of signaling molecules (NO and HO) in three types of tissue increased significantly. The magnitude of these results was higher with mixtures than with individual strains. These results imply that the two types of bacterial agents induce physiological metabolism changes to accumulate polysaccharides and lobetyolin by regulating stress resistance enzymes and signal molecules, especially NO and HO.
在这项研究中，我们专注于从党参中分离出的两株菌的促植物生长特性，以及不同菌株处理接种对党参生理和代谢产物积累的影响。菌株 RHBA19 和 RHBA17 分别被鉴定为地杆菌和荧光假单胞菌。这两株菌产生吲哚乙酸（IAA）、铁载体、生物膜和各种胞外酶。基于盆栽实验，接种 RHBA19（G 组）和两种混合菌（M 组）显著促进了党参的生长、根系发育和光合作用。与未接种组（CK）相比，G 组和 M 组的党参根总多糖含量分别显著提高了 59.27%和 96.07%，党参（根）的罗替醇含量分别提高了 58.4%和 66.0%。接种细菌剂后，党参抗氧化酶（CAT、POD、SOD）的活性差异增强。接种两种类型的细菌剂显著提高了党参根中蔗糖合酶（SS）和蔗糖磷酸合酶（SPS）以及叶片中苯丙氨酸解氨酶（PAL）的活性。此外，三种组织中的信号分子（NO 和 HO）含量显著增加。混合物的效果比单一菌株更高。这些结果表明，两种细菌剂通过调节应激抗性酶和信号分子（特别是 NO 和 HO）诱导生理代谢变化，从而积累多糖和罗替醇。
