School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China.
School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China.
J Plant Physiol. 2021 Dec;267:153546. doi: 10.1016/j.jplph.2021.153546. Epub 2021 Oct 23.
Codonopsis pilosula is a traditional Chinese herbal medicinal plant and contains various bioactive components, such as C. pilosula polysaccharides (CPPs) and lobetyolin (Lob). Hydrogen peroxide (HO) and nitric oxide (NO) are gaseous molecule and have been well known for their ability to relieve some adverse influences on plant from abiotic stress. Endophytic fungus is non-pathogenic plant-associated fungus that could play a significant role in improving plant tolerance by signal molecule. In this work, we determined how inoculation of Trichoderma strain RHTA01 with C. pilosula changed the plant's growth, metabolite accumulation, and related enzyme activity. Results demonstrated that application of Trichoderma strain RHTA01 significantly improved the growth of C. pilosula. Moreover, it noticeably decreased antioxidant enzyme superoxide dismutase (SOD) and catalase (CAT) activity in C. pilosula leaves, reduced the content of HO and malondialdehyde (MDA), and weakened the peroxidation of cell membrane lipids, which reduced the damage of abiotic stress to C. pilosula. Research has shown that it had obvious effects on levels of nitrogen and carbon metabolic enzymes. For example, sucrose synthase (SS) and acid invertase (AI) levels in C. pilosula roots were nearly 1.43 and 1.7 times higher, respectively, than those in the control (CK) group. In addition, it was notable that the production of CPPs and Lob, the most significant secondary metabolites in C. pilosula, were influenced by Trichoderma strain RHTA01. The obtained results indicate that inoculating C. pilosula with Trichoderma stimulates the carbon and nitrogen metabolism of the plant, and helps to increase the content of CPPs and Lob in the root of the plant.
党参是一种传统的中草药，含有多种生物活性成分，如党参多糖（CPPs）和 Lobetyolin（Lob）。过氧化氢（HO）和一氧化氮（NO）是气体分子，它们具有缓解植物非生物胁迫不良影响的能力而广为人知。内生真菌是一种非致病性的植物相关真菌，它可以通过信号分子在提高植物的耐受性方面发挥重要作用。在这项工作中，我们确定了用 Trichoderma 菌株 RHTA01 接种党参后，植物的生长、代谢产物积累和相关酶活性如何变化。结果表明，应用 Trichoderma 菌株 RHTA01 显著促进了党参的生长。此外，它明显降低了党参叶片中超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的抗氧化酶活性，降低了 HO 和丙二醛（MDA）的含量，减弱了细胞膜脂质的过氧化作用，从而减轻了非生物胁迫对党参的损伤。研究表明，它对氮碳代谢酶水平有明显影响。例如，党参根中蔗糖合酶（SS）和酸性转化酶（AI）的水平分别比对照（CK）组高 1.43 倍和 1.7 倍。此外，值得注意的是，Trichoderma 菌株 RHTA01 对 CPPs 和 Lob 的产生有影响，这是党参中最重要的次生代谢物。研究结果表明，用 Trichoderma 菌株接种党参刺激了植物的碳氮代谢，有助于提高植物根部 CPPs 和 Lob 的含量。
