Xie Min, Yan Zhiqiang, Ren Xia, Li Xiuzhuang, Qin Bo
CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences , Lanzhou 730000, PR China.
University of Chinese Academy of Sciences , Beijing 100049, China.
J Agric Food Chem. 2017 Mar 15;65(10):2032-2038. doi: 10.1021/acs.jafc.6b04320. Epub 2017 Mar 7.
Codonopilate A (1), a triterpenyl ester, was isolated from monocultivated soil of annual Codonopsis pilosula and identified as the main autotoxin. The yield ratio of codonopilate A in dried soil was calculated as 2.04 μg/g. Other two triterpenoids, taraxeryl acetate (2) and 24-methylenecycloartanol (3), were isolated and identified as well showing weaker autotoxity. This was the first time that the potential allelochemicals and autotoxins in the cultivated soil of Codonopsis pilosula were reported. Accumulation of reactive oxygen species (ROS) induced by the autotoxins in the root tips of Codonopsis pilosula was considered as an important factor for the phytotoxic effect. This work systematically investigates the allelopathic and autotoxic effect of Codonopsis pilosula, and the preliminary autotoxic action mode of the three autotoxins. These findings are helpful to understand the molecular mechanism of autotoxicity and conducive to explore proper ways to degrade the autotoxins and eliminate the replanting problems of Codonopsis pilosula.
党参酯A（1）是一种三萜酯，从一年生党参的单作土壤中分离得到，并被鉴定为主要的自毒物质。干燥土壤中党参酯A的产率为2.04μg/g。另外两种三萜类化合物，蒲公英赛醇乙酸酯（2）和24-亚甲基环阿尔廷醇（3）也被分离并鉴定出来，但其自毒作用较弱。这是首次报道党参栽培土壤中的潜在化感物质和自毒物质。党参根尖中自毒物质诱导的活性氧（ROS）积累被认为是造成植物毒性效应的一个重要因素。这项工作系统地研究了党参的化感和自毒作用，以及这三种自毒物质初步的自毒作用模式。这些发现有助于理解自毒作用的分子机制，并有利于探索降解自毒物质的合适方法，消除党参连作障碍问题。
