荧光标记作为一种评估聚合物纳米粒子在植物中摄取和转运的策略。

Fluorescent labeling as a strategy to evaluate uptake and transport of polymeric nanoparticles in plants.

机构信息

São Paulo State University (UNESP), Institute of Science and Technology of Sorocaba, Avenida Três de Março 511, Alto da Boa Vista, Sorocaba, São Paulo 18087-180, Brazil.

出版信息

Adv Colloid Interface Sci. 2022 Jul;305:102695. doi: 10.1016/j.cis.2022.102695. Epub 2022 May 13.

DOI:10.1016/j.cis.2022.102695

PMID:35598536

Abstract

The use of biodegradable nanopolymers in agriculture offers an excellent alternative for the efficient delivery of agrochemicals that promote plant protection and development. However, tracking of these systems inside plants requires complex probe tagging strategies. In addition to providing a basis for better understanding such nanostructures to optimize delivery system design, these probes allow monitoring the migration of nanoparticles through plant tissues, and determine accumulation sites. Thus, these probes are powerful tools that can be used to quantify and visualize nanoparticle accumulation in plant cells and tissues. This review is an overview of the methods involved in labeling nanocarriers, mainly based on polymeric matrices, for the delivery of nanoagrochemicals and the recent advances in this field.

摘要

在农业中使用可生物降解的纳米聚合物为高效传递农用化学品提供了极好的选择，这些化学品可以促进植物的保护和生长。然而，要追踪这些系统在植物体内的情况，则需要采用复杂的探针标记策略。除了为更好地了解这些纳米结构以优化传递系统设计提供基础外，这些探针还可以监测纳米颗粒通过植物组织的迁移情况，并确定其积累位置。因此，这些探针是功能强大的工具，可用于量化和可视化植物细胞和组织中纳米颗粒的积累。本综述概述了标记纳米载体（主要基于聚合物基质）以传递纳米农用化学品的方法，以及该领域的最新进展。