Liquid chromatographic determination of folic acid in multivitamin preparations.

A reversed-phase high-performance liquid chromatographic method is described for a stability-indicating assay of folic acid in multivitamin preparations. This method shows excellent recovery and precision characteristics, allows the determination of folic acid to levels of 0.1 microgram/mL, is simple to execute, and is suitable for high volume routine work. A good correlation was demonstrated between the HPLC method and the currently used bioassay.