McSharry W O, Mahn F P
J Pharm Sci. 1979 Feb;68(2):241-3. doi: 10.1002/jps.2600680233.
A collaborative study of the USP high-pressure liquid chromatographic assay for folic acid was performed. Two samples were analyzed in duplicate by 14 participating laboratories. Relative standard deviations for a single determination (RSDS) ranged from +/-0.40 to +/-2.39%. Based on an analysis of variance, it was concluded that the method of peak measurement was a major determinant of reproducibility and that graphical measurement was associated with a high standard deviation. Adequate resolution was obtained using a variety of columns and operating conditions. The interlaboratory RSDS was +/-1.8%.
开展了一项关于美国药典（USP）叶酸高效液相色谱测定法的协作研究。14个参与实验室对两个样品进行了双份分析。单次测定的相对标准偏差（RSDS）范围为±0.40%至±2.39%。基于方差分析得出结论，峰测量方法是重现性的主要决定因素，并且图形测量与高标准差相关。使用各种色谱柱和操作条件获得了足够的分离度。实验室间RSDS为±1.8%。
