Li Jia, Zhang Long
Plant Protection College, Shenyang Agricultural University, Shenyang, China.
College of Grassland Science and Technology, China Agricultural University, Beijing, China.
Arch Insect Biochem Physiol. 2022 Jul;110(3):e21911. doi: 10.1002/arch.21911. Epub 2022 May 22.
Odorants that bind well to odorant-binding proteins (OBPs) often trigger olfactory responses and have important biological significance. The locust Locusta migratoria (Meyen) (Orthoptera: Acrididae) is a serious agricultural pest. Twenty-one saturated aliphatic compounds with carbon-oxygen bonds and straight chains of 10-17 carbon atoms bind well to an L. migratoria OBP. In this study, olfactory activities of these aliphatic compounds on L. migratoria adult males were tested by electroantennography (EAG) and comparatively analyzed. Four alcohols (undecanol, dodecanol, tridecanol, and tetradecanol), two ketones (2-dodecanone and 2-tridecanone), and two esters (ethyl octanoate and ethyl nonanoate) triggered strong EAG responses, and there was no significant difference between them. The results suggest that the eight compounds are more likely to have important biological significance than the other compounds. Moreover, we found that there is not necessarily a positive correlation between the olfactory activity of odorants and their binding ability with OBP. The study contributes to understanding the odorants with biological significance for L. migratoria and the molecular mechanism of the locust's olfaction.
与气味结合蛋白（OBPs）结合良好的气味分子通常会引发嗅觉反应，并具有重要的生物学意义。飞蝗Locusta migratoria（Meyen）（直翅目：蝗科）是一种严重的农业害虫。21种具有碳 - 氧键且直链含有10 - 17个碳原子的饱和脂肪族化合物与一种飞蝗OBP结合良好。在本研究中，通过触角电图（EAG）测试了这些脂肪族化合物对飞蝗成年雄性的嗅觉活性，并进行了比较分析。四种醇（十一醇、十二醇、十三醇和十四醇）、两种酮（2 - 十二酮和2 - 十三酮）以及两种酯（辛酸乙酯和壬酸乙酯）引发了强烈的EAG反应，且它们之间没有显著差异。结果表明，这八种化合物比其他化合物更有可能具有重要的生物学意义。此外，我们发现气味分子的嗅觉活性与其与OBP的结合能力之间不一定存在正相关。该研究有助于理解对飞蝗具有生物学意义的气味分子以及蝗虫嗅觉的分子机制。
