Li Jia, Zhang Long, Wang Xiaoqi
Plant Protection College, Shenyang Agricultural University, Shenyang, China.
Key Lab for Biological Control, China Agricultural University, Beijing, China.
Arch Insect Biochem Physiol. 2016 Apr;91(4):221-9. doi: 10.1002/arch.21319. Epub 2016 Feb 10.
Locusts, Locusta migratoria (Orthoptera: Acrididae), are extremely destructive agricultural pests, but very little is known of their molecular aspects of perception to host plant odorants including related odorant-binding proteins (OBPs), though several OBPs have been identified in locust. To elucidate the function of LmigOBP1, the first OBP identified from locust, RNA interference was employed in this study to silence LmigOBP1, which was achieved by injection of dsRNA targeting LmigOBP1 into the hemolymph of male nymphs. Compared with LmigOBP1 normal nymphs, LmigOBP1 knockdown nymphs significantly decreased food (maize leaf, Zea mays) consumption and electro-antennography responses to five maize leaf volatiles, ((Z)-3-hexenol, linalool, nonanal, decanal, and (Z)-3-hexenyl acetate). These suggest that LmigOBP1 is involved in perception of host plant odorants.
飞蝗（Locusta migratoria，直翅目：蝗科）是极具破坏性的农业害虫，尽管已在飞蝗中鉴定出几种气味结合蛋白（OBP），但对于它们感知寄主植物气味分子的分子机制，包括相关的气味结合蛋白，人们了解甚少。为阐明从飞蝗中鉴定出的首个OBP——LmigOBP1的功能，本研究采用RNA干扰技术使LmigOBP1沉默，通过将靶向LmigOBP1的双链RNA注射到雄性若虫的血淋巴中来实现。与LmigOBP1正常的若虫相比，LmigOBP1基因敲低的若虫对食物（玉米叶，Zea mays）的消耗量以及对五种玉米叶挥发物（（Z）-3-己烯醇、芳樟醇、壬醛、癸醛和（Z）-3-己烯基乙酸酯）的触角电位反应均显著降低。这些结果表明，LmigOBP1参与了对寄主植物气味分子的感知。
