Jiang Xin, Qin Yaoguo, Jiang Jun, Xu Yun, Francis Frédéric, Fan Jia, Chen Julian
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Functional and Evolutionary Entomology, Gembloux Agro-Bio Tech, University of Liège, Gembloux, Belgium.
Front Physiol. 2022 May 4;13:877133. doi: 10.3389/fphys.2022.877133. eCollection 2022.
In China, is one of the most common endoparasitoids of the green peach aphid and grain aphid in the field. Insect odorant-binding proteins (OBPs) play vital roles in odor perception during feeding, host searching, mating and oviposition. In addition, some OBPs are involved in other physiological processes such as gustation and reproduction. In the present study, a comparative antennal transcriptomic analysis was applied between male and female . The spatial expression patterns among antennae, heads, thoraxes, abdomens and legs of OBPs in both sexes were further profiled. Fifteen s were predicted, and 14 of them were identified by gene cloning, including 12 classic OBPs and 2 min-C OBPs. As expected, all OBPs were mainly expressed at high levels in antennae, heads or legs which are sensory organs and tissues. Finally, ligand binding properties of 2 OBPs (AgifOBP7 and AgifOBP9) were further evaluated. Female leg specifically expressed AgifOBP9 displays a broad and high binding property to aphid alarm pheromones, plant green volatiles and aphid sex pheromones (Ki < 10 μΜ). However, female leg specifically expressed AgifOBP7 displays poor affinity for all tested ligands except CAU-II-11 ((E)-3,7-dimethylocta-2,6-dien-1-yl-2-hydroxy-3-methoxybenzoate), a reported (E)-β-farnesene (EBF) analog with an exceptionally high binding affinity (Ki = 1.07 ± 0.08 μΜ). In summary, we reported the spatial expression pattern of the OBP repertoire in , and further studied the binding properties of OBP7 and OBP9, which are mainly expressed in female legs, laying the foundation for the dissection of the contribution of OBPs to chemosensation in .
在中国，它是田间桃蚜和麦长管蚜最常见的内寄生蜂之一。昆虫气味结合蛋白（OBPs）在取食、寄主搜索、交配和产卵期间的气味感知中起着至关重要的作用。此外，一些OBPs还参与其他生理过程，如味觉和繁殖。在本研究中，对该蜂的雌雄个体进行了触角转录组比较分析。进一步分析了两性OBPs在触角、头部、胸部、腹部和腿部的空间表达模式。预测了15个OBPs，其中14个通过基因克隆得到鉴定，包括12个经典OBPs和2个微型C OBPs。正如预期的那样，所有OBPs主要在触角、头部或腿部等感觉器官和组织中高水平表达。最后，进一步评估了2个OBPs（AgifOBP7和AgifOBP9）的配体结合特性。雌性腿部特异性表达的AgifOBP9对蚜虫报警信息素、植物绿色挥发物和蚜虫性信息素具有广泛且高的结合特性（Ki < 10 μΜ）。然而，雌性腿部特异性表达的AgifOBP7对所有测试配体的亲和力较差，除了CAU-II-11（(E)-3,7-二甲基辛-2,6-二烯-1-基-2-羟基-3-甲氧基苯甲酸酯），一种报道的具有异常高结合亲和力（Ki = 1.07 ± 0.08 μΜ）的(E)-β-法尼烯（EBF）类似物。总之，我们报道了该蜂OBP库的空间表达模式，并进一步研究了主要在雌性腿部表达的OBP7和OBP9的结合特性，为剖析OBPs对该蜂化学感受的贡献奠定了基础。
