State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China.
Curr Biol. 2022 Mar 14;32(5):951-962.e7. doi: 10.1016/j.cub.2021.12.054. Epub 2022 Jan 21.
(E)-β-farnesene (EBF) is an important chemical cue mediating interactions between plants, aphids, and natural enemies. This chemical has two origins, being secreted by aphid as an alarm pheromone and also produced by the attacked plants as a semiochemical attracting natural enemies. Despite the important role of this volatile chemical, little is known on the molecular mechanisms mediating the attraction of natural enemies to EBF. Here, we first verified that the larvae and adults of aphid predator hoverfly Eupeodes corollae detect and are attracted to EBF. Then, we found a neuron housed in type III basiconic sensilla of adult antenna responding to EBF. We further verified that in both adults and larvae odorant receptor EcorOR3 and odorant-binding protein EcorOBP15 mediate detection of EBF and structurally similar volatiles. Finally, we provide evidence that larvae of E. corollae may use aphid-derived EBF for prey location in the short-range, whereas adults could detect plant-derived EBF to find attacked plants from longer distances. Thus, while dissecting the molecular basis for attraction to EBF produced by two different sources, our results may find potential applications in integrated aphid management approaches.
(E)-β-法呢烯（EBF）是一种重要的化学线索，介导植物、蚜虫和天敌之间的相互作用。这种化学物质有两个来源，即蚜虫作为报警信息素来分泌，以及被攻击的植物作为吸引天敌的半化学物质来产生。尽管这种挥发性化学物质具有重要作用，但对于介导天敌对 EBF 的吸引力的分子机制知之甚少。在这里，我们首先验证了蚜虫捕食性蝇类草蛉 Eupeodes corollae 的幼虫和成虫可以检测到并被 EBF 吸引。然后，我们发现了一种存在于成年触角 III 型锥形感器中的神经元对 EBF 作出反应。我们进一步验证了在成虫和幼虫中，气味受体 EcorOR3 和气味结合蛋白 EcorOBP15 介导对 EBF 和结构相似的挥发物的检测。最后，我们提供了证据表明，草蛉幼虫可能在短距离内使用蚜虫产生的 EBF 来定位猎物，而成虫则可以从更远的距离检测到植物产生的 EBF 来找到被攻击的植物。因此，在剖析两种不同来源的 EBF 吸引力的分子基础的同时，我们的研究结果可能为综合蚜虫管理方法找到潜在的应用。
