Center of Liver Diseases Division 3, Beijing Ditan Hospital, Capital Medical University, Beijing, China.
National Center for Infectious Diseases, Beijing, China.
J Diabetes Res. 2022 May 13;2022:1826181. doi: 10.1155/2022/1826181. eCollection 2022.
Chronic hepatitis B cirrhosis is often accompanied by glucose metabolism disorder, and intestinal microbiota was closely related to both cirrhosis and diabetes. There are few studies on the role of intestinal microbiota in hepatitis B liver cirrhosis and diabetes mellitus (LCDM). The purpose of this study was to investigate the characteristics of intestinal microbiota in patients with LCDM and to evaluate the relationship between the severity of intestinal microbiota imbalance and clinical significance.
A case-controlled study was conducted. People who met the inclusion and exclusion criteria of chronic HBV-related liver cirrhosis (LC), LCDM, and healthy controls (HC) were enrolled in, and their fecal and blood samples were collected. The V3-V4 region of 16s rDNA gene of fecal microbiota was sequenced; the bioinformatics analysis including -diversity, -diversity, and linear discriminant analysis (LDA) effect size (LEfSe) was performed; and the correlation between bacteria and clinical indexes was analyzed.
A total of 70 participants completed fecal and blood tests, including 20 HC, 20 LCDM, and 30 LC. The diversity of intestinal microbiota in the LCDM decreased than that in the HC. The abundance of , , , and increased, while the abundance of , , , , and decreased in the LCDM compared with the HC. The abundance of , , and and the degree of hepatitis B cirrhosis dysbiosis indicator (HBCDI) increased in the LCDM than in the LC. The abundance of , , and positively correlated with liver injury and fasting blood glucose (FBG) level. The abundance of , , , and increased more significantly when FBG and glycosylated hemoglobin level increased.
Intestinal microbiota of patients with LCDM was significantly disordered, and the degree was more serious than that cirrhosis patients without diabetes.
慢性乙型肝炎肝硬化常伴有糖代谢紊乱，而肠道微生物群与肝硬化和糖尿病都密切相关。关于肠道微生物群在乙型肝炎肝硬化和糖尿病（LCDM）中的作用的研究较少。本研究旨在探讨 LCDM 患者肠道微生物群的特征，并评估肠道微生物群失衡严重程度与临床意义的关系。
采用病例对照研究，纳入符合慢性 HBV 相关肝硬化（LC）、LCDM 和健康对照（HC）纳入标准和排除标准的患者，并采集其粪便和血液样本。对粪便微生物群的 16s rDNA 基因 V3-V4 区进行测序；进行生物信息学分析，包括 -多样性、-多样性和线性判别分析（LDA）效应量（LEfSe）；分析细菌与临床指标的相关性。
共 70 名参与者完成了粪便和血液检查，包括 20 名 HC、20 名 LCDM 和 30 名 LC。LCDM 患者肠道微生物群的多样性低于 HC。与 HC 相比，LCDM 中 、 、 、 的丰度增加，而 、 、 、 的丰度降低。与 LC 相比，LCDM 中 、 、 和 HBCDI 增加。 、 、 和 的丰度与肝损伤和空腹血糖（FBG）水平呈正相关。 、 、 和 的丰度随 FBG 和糖化血红蛋白水平的升高而增加更为显著。
LCDM 患者的肠道微生物群明显失调，且严重程度较无糖尿病的肝硬化患者更严重。
