Ahmadnejad-Asl-Gavgani Masoud, Maham Masoud, Dalair-Naghadeh Bahram
DVSc Candidate, Department of Internal Medicine and Clinical Pathology, Faculty of Veterinary Medicine, Urmia University , Urmia, Iran.
Department of Internal Medicine and Clinical Pathology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Vet Res Forum. 2022 Mar;13(1):29-37. doi: 10.30466/vrf.2021.521204.3118. Epub 2022 Mar 15.
() is a medicinal plant previously used in human medicine to solve gastrointestinal problems such as abdominal pain. Anti-inflammatory, analgesic, immune-modulatory, and antioxidant effects of have been proven in previous studies. The present study investigated the effects of essential oil (TBEO) on ruminant smooth muscle contractions. The experiment was performed on the circular smooth muscle of ileum samples taken from slaughtered bulls in the organ bath. Nine cumulative concentrations of TBEO from 0.10 to 1000 µg mL were added to tissue samples. The solution used was Tyrode's solution aerated with a mixture of 95.00% oxygen and 5.00% carbon dioxide, and the temperature was set at 37.00 ˚C. The effect of TBEO on baseline contractions and three induced contractions with potassium chloride, barium chloride, and carbachol was investigated. In GC-MS analysis of TBEO, carvone was identified as a major ingredient. The effects of eight concentrations of 0.001 to 10.00 µM of carvone on all contractions were investigated under similar conditions. The effects of TBEO, carvone, and verapamil (standard calcium channel blocker) on calcium channels were assessed. The results revealed that TBEO and carvone significantly inhibit spontaneous contractions as well as all spasmogen-induced contractions. The TBEO and carvone exert their myorelaxant properties by inhibiting Ca channels in smooth muscle. The anti-spasmodic properties of can be employed for the treatment of intestinal spasms or hypermotility.
（）是一种曾用于人类医学以解决诸如腹痛等胃肠道问题的药用植物。先前的研究已证实其具有抗炎、镇痛、免疫调节和抗氧化作用。本研究调查了（）精油（TBEO）对反刍动物平滑肌收缩的影响。实验在器官浴中取自屠宰公牛的回肠环形平滑肌样本上进行。将九种浓度从0.10至1000 µg/mL累积的TBEO添加到组织样本中。所使用的溶液是用95.00%氧气和5.00%二氧化碳的混合物曝气的台氏液，温度设定为37.00˚C。研究了TBEO对基线收缩以及用氯化钾、氯化钡和卡巴胆碱诱导的三种收缩的影响。在TBEO的气相色谱 - 质谱分析中，香芹酮被鉴定为主要成分。在相似条件下研究了八种浓度从0.001至10.00 µM的香芹酮对所有收缩的影响。评估了TBEO、香芹酮和维拉帕米（标准钙通道阻滞剂）对钙通道的影响。结果显示，TBEO和香芹酮显著抑制自发收缩以及所有致痉剂诱导的收缩。TBEO和香芹酮通过抑制平滑肌中的钙通道发挥其肌松作用。（）的抗痉挛特性可用于治疗肠痉挛或肠道运动亢进。
